食品中细菌分型方法综述  

A review of bacterial typing methods in food

作　　者：王庆平 薛晓晶[1] 朱萧燕[1] 李玲 孙金红 WANG Qingping;XUE Xiaojing;ZHU Xiaoyan;LI Ling;SUN Jinhong(Chinese Academy of Inspection and Quarantine Comprehensive Test Center,Beijing,100123,China)

机构地区：[1]中国检验检疫科学研究院综合检测中心,北京100123

出　　处：《质量安全与检验检测》2024年第3期22-29,共8页QUALITY SAFETY INSPECTION AND TESTING

基　　金：中国检验检疫科学研究院基本科研业务费项目(2022JK22)。

摘　　要：细菌分型技术可用于食品中污染菌的定性和溯源,以及食品中益生菌质量控制和监管。本文对目前食品检测中常用的细菌分型研究方法的原理、应用及优缺点进行了阐述,主要包括基于分子生物学方法的脉冲场凝胶电泳(PFGE)、限制性片段长度多态性(RFLP)、扩增片段长度多态性(AFLP)、随机增多态性DNA(RAPD)、多位点序列分型(MLST)、多位点可变数目串联重复分析(MLVA)以及基于质谱及光谱技术的基质辅助激光解吸电离飞行时间质谱(MALDI-TOF MS)、傅里叶变换红外光谱(FTIR)等技术。Bacterial typing technology can be used for qualitative and traceability of contaminated bacteria in food,as well as quality control and supervision of probiotics in food.In this paper,the principles,applications,advantages and disadvantages of commonly used research methods for bacterial typing in food detection were reviewed,including pulsed field gel electrophoresis(PFGE),restriction fragment length polymorphism(RFLP),amplified fragment length polymorphism(AFLP),randomly amplified polymorphic DNA(RAPD),multi site sequence typing(MLST),multi site variable number tandem repeat analysis(MLVA)based on molecular biology methods,matrix assisted laser desorption ionization time of flight mass spectrometry(MALDI-TOF MS),Fourier transform infrared spectroscopy(FTIR)and other technologies based on mass spectrometry and spectroscopy.

关 键 词：细菌分型 分子检测 质谱光谱技术 

分 类 号：Q93-31[生物学—微生物学]