高效液相色谱法测定复方黄番口服液中芍药苷含量  

Content Determination of Paeoniflorin in Compound Huangfan Oral Liquid by HPLC

作　　者：张育勤 米婷婷 赵俊红 辜燕茹 王曦叶 ZHANG Yuqin;MI Tingting;ZHAO Junhong;GU Yanru;WANG Xiye(General Hospital of Western Theater Command of the PLA,Chengdu,Sichuan,China 610083;School of Pharmacy,North Sichuan Medical College,Nanchong,Sichuan,China 637000)

机构地区：[1]中国人民解放军西部战区总医院,四川成都610083 [2]川北医学院药学院,四川南充637000

出　　处：《中国药业》2024年第13期74-76,共3页China Pharmaceuticals

摘　　要：目的建立测定复方黄番口服液中芍药苷含量的高效液相色谱法。方法色谱柱为Agilent Eclipse Plus C_(18)柱(150 mm×4.6 mm,5µm),流动相为甲醇-0.1%磷酸溶液(26∶74,V/V),流速为1.0 mL/min,检测波长为230 nm,柱温为30℃,进样量为10µL。结果芍药苷的质量浓度在15.35~168.85µg/mL范围内与峰面积线性关系良好(r=0.9996,n=6);精密度、稳定性、重复性试验结果的RSD均小于1.0%(n=6);平均回收率为95.03%,RSD为0.78%(n=9)。结论该方法结果准确、稳定性和重复性均良好,可用于复方黄香口服液中芍药苷的含量测定。Objective To establish a high-performance liquid chromatography(HPLC)method for the content determination of paeoniflorinof in Compound Huangfan Oral Liquid.Methods The chromatography column was Agilent Eclipse Plus C_(18) column(150 mm×46 mm,5µm),the mobile phase was methanol-0.1%phosphoric acid solution(26∶74,V/V),the flow rate was 1.0 mL/min,the detection wavelength was 230 nm,the column temperature was 30℃,and the injection volume was 10µL.Results The linear range of paeoniflorin was 15.35-168.85µg/mL(r=0.9996,n=6).The RSDs of precision,stability,and repeatability test results were all lower than 1.0%(n=6).The average recovery of paeoniflorin was 95.03%with an RSD of 0.78%(n=9).Conclusion The method is accurate,stable,and has good repeatability,which can be used for the content determination of paeoniflorin in Compound Huangfan Oral Liquid.

关 键 词：高效液相色谱法 复方黄番口服液 芍药苷 含量测定 

分 类 号：R932[医药卫生—生药学] R284.1[医药卫生—药学] R286.0