国内部分动物园与花鸟市场鹦鹉热衣原体病原学检测  

Pathogenic Detection of Chlamydia psittaci in Zoos and Flower-bird Markets in China

作　　者：刘心仪 包银莉 张释丹 陆浩天 文洪然 陈柳蓉 张钲浩 李金飞 陈蓉 刘燕[5] 代蕾 刘广锦[1] Liu Xinyi;BaoYinli;Zhang Shidan;Lu Haotian;Wen Hongran;Chen Liurong;Zhang Zhenghao;Li Jinfei;Chen Rong;Liu Yan;Dai Lei;Liu Guangjin(Sanya Institute of Nanjing Agricultural University,Sanya 572025,Hainan,China;Longyan College,Longyan 364012,Fujian,China;Nanjing Agricultural University,Nanjing 210095,Jiangsu,China;Nanjing Hongshan Forest Zoo,Nanjing 210028,Jiangsu,China;Beijing Zoo,Beijing 100044,China;Hainan Center for Animal Disease Prevention and Control,Haikou 570203,Hainan,China)

机构地区：[1]南京农业大学三亚研究院,海南三亚572025 [2]龙岩学院,福建龙岩364012 [3]南京农业大学,江苏南京210095 [4]南京红山森林动物园,江苏南京210028 [5]北京动物园,北京100044 [6]海南省动物疫病预防与控制中心,海南海口570203

出　　处：《中国动物检疫》2024年第6期50-55,共6页China Animal Health Inspection

基　　金：南京农业大学三亚研究院引导资金项目(NAUSY-MS21);海南省重点研发项目(ZDYF2022XDNY236);北京动物园圈养野生动物技术北京市重点实验室项目(ZDK202202)。

摘　　要：为了解国内动物园与花鸟市场鸟群中鹦鹉热衣原体(Chlamydia psittaci,Cps)的流行现状,通过构建TaqMan荧光定量PCR方法,对江苏省和北京市3处采样点采集的168份禽鸟粪便样本进行了检测,并基于OmpA基因对阳性样本进行了基因序列分析。结果显示:在江苏省1处花鸟市场检出31份Cps阳性,个体阳性率为41.3%(31/75),在其他2处动物园采样点未检出阳性;阳性样品来自虎皮鹦鹉(23份)、牡丹鹦鹉(5份)、玄凤鹦鹉(2份)和珍珠鸟(1份)。在阳性样本中,共扩增出18个OmpA基因片段,其中16份样本的基因序列相同,与另外2份阳性样本序列不在同一分支,但所有阳性样本均属于基因A型。结果表明,花鸟市场存在较高的Cps流行风险,感染源较为集中。结果提示,各地应着重加强花鸟市场Cps流行的防控,严格落实相关监管措施,加强检疫监管,从而降低Cps传播率并保障公共卫生安全。In order to investigate the prevalence of Chlamydia psittaci(Cps)in bird flocks in some zoos and flowerbird markets in China,168 avian fecal samples collected from three sampling sites in Jiangsu Province and Beijing were detected using TaqMan fluorescence quantitative PCR,and gene sequence analysis was conducted for positive samples based on OmpA gene.The results showed that 31 Cps-positive samples were detected in a flower-bird market in Jiangsu Province,with an individual positive rate of 41.3%(31/75),but none was in the other two sampling sites(zoos);the positive samples were from budgerigars(23),peafowl parrots(5),gentoo parrots(2)and perching birds(1).For the positive samples,18 OmpA gene fragments were amplified,in which 16 samples had the same gene sequence,that were not in the same branch as the gene sequence in other two positive samples,but all of which belonged to genotype A.In conclusion,the risk of Cps prevalence in the flower-bird markets was relatively high,with concentrated sources of infection.Cps should be further prevented and controlled in local flower-bird markets,relevant regulatory measures should be implemented to improve quarantine supervision,aiming to reduce its transmission rate and safeguard public health safety.

关 键 词：鹦鹉热衣原体 核酸检测 qPCR 基因进化树 遗传分析 

分 类 号：S851.3[农业科学—预防兽医学]