低温胁迫下6种小檗属植物生理特性及耐寒性评价  

Evaluation of the Physiological Characteristics and Cold Tolerance of Six Berberis Under Low Temperature Stress

作　　者：田新格 何振华 顾文毅[1] 祁银燕 Tian Xinge;He Zhenhua;Gu Wenyi;Qi Yinyan(Academy of Agriculture and Forestry Sciences,Qinghai University/Qinghai Academy of Agriculture and Forestry Sciences,Tree Genetics and Breeding Laboratory of Qinghai Plateau,Key Laboratory of Tree Genetics and Breeding of Qinghai Plateau,National Forestry and Grassland Administration,Xining 810016,China;Xunhua Salar Autonomous County Natural Resources and Forestry Grassland Bureau,Haidong 811100,China;Research and Utilization Laboratory of Qinghai-Tibet Plateau Germplasm Resources,Xining 810016,China)

机构地区：[1]青海大学农林科学院/青海省农林科学院青海省高原林木遗传育种重点实验室,青海高原林木遗传育种国家林业和草原局重点实验室,西宁810016 [2]循化撒拉族自治县自然资源和林业草原局,海东811100 [3]青藏高原种质资源研究与利用实验室,西宁810016

出　　处：《青海科技》2024年第2期161-168,共8页Qinghai Science and Technology

摘　　要：以青海省6种小檗属植物的休眠枝条为试材,采用人工梯度降温的方法,分别在-20℃、-25℃、-30℃、-35℃、-40℃下处理24 h,以4℃为对照。研究了低温处理后各小檗枝条各项生理指标。结果表明:随着处理温度的降低,拟小檗的相对电导率呈持续下降趋势,鲜黄小檗的相对电导率呈持续上升趋势,刺檗、直穗小檗、匙叶小檗、细叶小檗的相对电导率呈先上升后下降趋势;拟小檗、匙叶小檗PRO含量呈持续上升趋势,刺檗、鲜黄小檗、直穗小檗、细叶小檗PRO含量呈持续下降趋势;MDA、POD、SOD、SS、SP变化一致,呈先上升后下降趋势。通过计算各单项指标的抗寒系数,建立隶属函数进行评价,评价结果为鲜黄小檗﹥直穗小檗﹥刺檗﹥拟小檗﹥匙叶小檗﹥细叶小檗。The dormant branches of six Berberis plants in Qinghai Province were used as test materials,and the artificial gradient cooling method was used to treat them at-20°C,-25°C,-30°C,-35°C,-40°C for 24 h,with 4℃ as the control.The physiological indexes of Berberis branches after low temperature treatment were studied.The results showed that with the decrease of treatment temperature,the relative conductivity of Berberis dubia showed a continuous downward trend,the relative conductivity of Berberis diaphana showed a continuous upward trend,and the relative conductivity of Berberis vulgaris,Berberis dasystachya,Berberis vernae and Berberis poiretii showed a trend of increasing first and then decreasing.The PRO content of Berberis dubia and Berberis vernae showed a continuous upward trend,while the PRO content of Berberis vulgaris,Berberis diaphana,Berberis dasystachya and Berberis poiretii showed a continuous downward trend.The changes of MDA,POD,SOD,SS and SP were consistent,showing a trend of increasing first and then decreasing.By calculating the cold resistance coefficient of each single index,the membership function is established for evaluation.The evaluation results were as follows:Berberis diaphana>Berberis dasystachya>Berberis vulgaris>Berberis dubia>Berberis vernae>Berberis poiretii.

关 键 词：小檗 人工低温胁迫 隶属函数法 

分 类 号：S727.22[农业科学—林木遗传育种]