Development and parentage analysis of SNP markers for Chestnut-vented Nuthatch(Sitta nagaensis)based on ddRAD-seq data  

作　　者：Qingmiao Yuan Xi Lu Ruixin Mo Xianyin Xu Xu Luo Yubao Duan 

机构地区：[1]Key Laboratory for Conserving Wildlife with Small Populations in Yunnan,Southwest Forestry University,Kunming 650224,China [2]Key Laboratory for Forest Resources Conservation and Utilization in the Southwest Mountains of China,Ministry of Education,Southwest Forestry University,Kunming 650224,China [3]Key Laboratory of Forest Disaster Warning and Control of Yunnan Province,Southwest Forestry University,Kunming 650224,China [4]College of Forestry,Southwest Forestry University,Kunming 650224,China [5]Administration of Zixi Mountain Provincial Nature Reserve,Chuxiong 675008,China

出　　处：《Avian Research》2024年第2期271-278,共8页鸟类学研究（英文版）

基　　金：supported by the Science and Technology Project of Yunnan(202101AT070040);the National Natural Science Foundation of China(U23A20162);the Yunnan Provincial Department of Education Fund for Scientific Research Project(2023Y0722);the First Class Forestry Academic Subject in Yunnan Province.

摘　　要：Extra-pair paternity(EPP)is commonly found in socially monogamous birds,especially in small passerine birds,and there are interspecific and intraspecific variations in the extent of EPP.The Chestnut-vented Nuthatch(Sitta nagaensis)is a socially monogamous passerine bird,and verifying whether this species has EPP relies on parentage testing-S.nagaensis is not known to have EPP.In this study,we developed SNP markers of this species that are informative for parentage analysis from double digest restriction site-associated DNA sequencing(ddRAD-seq)data.A panel consisting of 50 SNP markers,with a mean heterozygosity of 0.343,was used to resolve 95% of nestlings to fathers.The combined exclusion probabilities for the first parent and second parent were 0.991 and 0.9999,respectively.This panel of SNP markers is a powerful tool for parentage assignments in S.nagaensis.In addition,we found that three offspring(7.9%)from three nests(23.1%)were the result of extra-pair fertilization out of 38 offspring in 13 nests.Our study provided information on parentage analysis that has not been reported before in S.nagaensis.It also supplemented the understudied EPP behavior of birds in Asia,contributing to a general understanding of the EPP behaviors of birds.

关 键 词：Chestnut-vented Nuthatch ddRAD sequencing Extra-pair paternity Parentage analysis SNPS 

分 类 号：Q953[生物学—动物学]