HBV感染患者外周血ISGs表达水平变化及其与干扰素治疗疗效的关系  

Changes of peripheral blood ISGs and their relationship with curative effect of interferon therapy in patients with HBV infection

作　　者：胡静 杨秀锦 侯志云 Hu Jing;Yang Xiujin;Hou Zhiyun(Department of Infectious Disease,Daxing Teaching Hospital of Capital Medical University,Beijing 102600,China;Department of Emergency,Shijingshan Teaching Hospital of Capital Medical University,Beijing 100040,China)

机构地区：[1]首都医科大学大兴教学医院感染内科,北京102600 [2]首都医科大学石景山教学医院急诊科,北京100040

出　　处：《中华消化病与影像杂志（电子版）》2024年第4期343-347,共5页Chinese Journal of Digestion and Medical Imageology(Electronic Edition)

摘　　要：目的探讨HBV感染患者外周血干扰素刺激基因(ISGs)表达水平变化及其与干扰素治疗疗效的关系。方法选取首都医科大学大兴教学医院2018年4月至2023年5月收治的80例HBV感染患者纳入HBV组,另选取同期进行健康体检者80例作为对照组。对比两组外周血黏病毒抗性蛋白A(MxA)mRNA、泛素特异性蛋白酶18(USP18)mRNA、细胞因子信号转导抑制因子3(SOCS3)mRNA表达水平,HBV组患者均给予重组人干扰素α-2b持续治疗24周,依照干扰素应答情况,分为干扰素应答组(n=38)与干扰素无应答组(n=42)两个亚组,通过Logistic回归分析外周血指标与干扰素应答的关系,并观察HBV组患者干扰素治疗前后MxA mRNA、USP18 mRNA、SOCS3 mRNA水平变化。结果HBV组治疗前外周血MxA mRNA水平低于对照组,USP18 mRNA、SOCS3 mRNA水平高于对照组(P<0.05);干扰素应答组治疗前外周血MxA mRNA水平高于干扰素无应答组,USP18 mRNA、SOCS3 mRNA水平低于干扰素无应答组(P<0.05);Logistic回归分析显示USP18 mRNA、SOCS3 mRNA高表达是HBV感染患者干扰素无应答危险因素,MxA mRNA高表达是干扰素应答保护因素;干扰素应答组治疗前、治疗2周、12周、24周后外周血MxA mRNA表达水平高于干扰素无应答组,USP18 mRNA、SOCS3 mRNA表达水平低于干扰素无应答组(P<0.05)。结论HBV感染者应用干扰素治疗中,MxA高表达、USP18、SOCS3低表达者具有较佳的干扰素应答疗效。Objective To explore the changes of peripheral blood interferon-stimulated genes(ISGs)and their relationship with curative effect of interferon therapy in patients with hepatitis B virus(HBV)infection.Methods A total of 80 patients with HBV infection and 80 healthy controls in Daxing Teaching Hospital of Capital Medical University were enrolled as HBV group and control group between April 2018 and May 2023,respectively.The mRNA levels of myxovirus resistance protein A(MxA),ubiquitin-specific protease 18(USP18)and suppressors of cytokine signaling 3(SOCS3)in peripheral blood were compared between the two groups.The patients in the HBV group were treated with recombinant human interferonα-2b for 24 weeks.According to interferon response,they were divided into response group(n=38)and non-response group(n=42).The relationship between peripheral blood indexes and interferon response was analyzed by Logistic regression analysis,and changes of MxA mRNA,USP18 mRNA and SOCS3 mRNA before and after interferon treatment in the HBV group were observed.Results Before treatment,level of peripheral blood MxA mRNA in the HBV group was lower than that in the control group,while levels of USP18 mRNA and SOCS3 mRNA were higher than those in the control group(P<0.05).Before treatment,level of peripheral blood MxA mRNA in the response group was higher than that in the non-response group,while levels of USP18 mRNA and SOCS3 mRNA were lower than those in the non-response group(P<0.05).Logistic regression analysis showed that high expressions of USP18 mRNA and SOCS3 mRNA were risk factors of interferon non-response,while high expression of MxA mRNA was a protective factor of interferon response.Before treatment,after 2 weeks,12 weeks and 24 weeks of treatment,level of peripheral blood MxA mRNA in the interferon response group was higher than that in the non-response group,while levels of USP18 mRNA and SOCS3 mRNA were lower than those in the non-response group(P<0.05).Conclusion In the treatment of HBV infection with interferon,interfer

关 键 词：乙型肝炎病毒 干扰素 干扰素刺激基因 治疗应答 

分 类 号：R512.62[医药卫生—内科学]