圆窗膜挑开前后豚鼠听功能变化研究  

Effect of Opening Round Window Membrane on Auditory Function of Healthy Guinea Pigs

作　　者：张斯 王林娥[1] 刘攀[1] 郑文蕊 朱桢 段蓓蕾 ZHANG Si;WANG Lin-e;LIU Pan;ZHENG Wen-rui;ZHU Zhen;DUAN Bei-lei

机构地区：[1]首都医科大学附属北京友谊医院耳鼻咽喉头颈外科,北京100050

出　　处：《中国听力语言康复科学杂志》2024年第4期433-437,446,共6页Chinese Scientific Journal of Hearing and Speech Rehabilitation

基　　金：北京市科学技术委员会“应用技术研究与开发项目”(Z191100007619043)。

摘　　要：目的探讨圆窗膜挑开前后健康豚鼠听功能的变化。方法选取健康豚鼠30只(60耳),吸入异氟烷麻醉后打开听泡挑开圆窗膜挑开前后分别行听性脑干反应(ABR)、耳蜗电图(electrocochleogram,ECochG)、电诱发复合动作电位(electrically evoked compound action potentials,ECAP)及电诱发听性脑干反应(electrically evoked auditory brainstem responses,EABR)检测,进行统计学分析。结果豚鼠54耳记录到分化良好且稳定的以III波为主的ABR、ECochG、ECAP及EABR波形。圆窗膜挑开前后ABR阈值分别为36.67±7.76 dB SPL、98.81±10.74 dB SPL,二者之间有显著差异(P<0.05);ECochG中CAP阈值分别为28.73±9.72 dB SPL、68.48±11.21 dB SPL,二者之间有显著差异(P<0.05);ECAP阈值分别为0.35±0.11 mA、0.46±0.13 mA,二者之间有显著差异(P<0.05);EABR阈值分别为0.21±0.05 mA、0.30±0.07 mA,二者之间有显著差异(P<0.05)。结论圆窗膜挑开前后健康豚鼠的听功能出现明显变化,表现为听力下降。Objective To investigate the effect of opening round window membrane on auditory function in healthy guinea pigs.Method Thirty healthy guinea pigs(60 ears)were anesthetized by inhalation of isoflurane.Otocyst was opened and round window film was opened.Auditory brainstem response(ABR),electrocochleogram(ECochG),electrically evoked auditory brainstem responses(EABR)and electroevoked compound action potentials(ECAP)was recorded before and after the round window membrane was opened.And statistical analysis was performed.Results In 54 ears of guinea pigs,well-differentiated and stable waveforms of ABR,ECochG,EABR and ECAP were recorded.The ABR thresholds before and after opening round window membrane were 36.67±7.76 dB SPL and 98.81±10.74 dB SPL,the difference between them was statistically significant(P<0.05).The ECochG thresholds before and after opening round window membrane were 28.73±9.72 dB SPL and 68.48±11.21 dB SPL,the difference between them was statistically significant(P<0.05);The EABR thresholds before and after opening round window membrane were 0.21±0.05 mA and 0.30±0.07 mA,and the difference between them was statistically significant(P<0.05);The ECAP thresholds before and after opening round window membrane were 0.35±0.11 mA and 0.46±0.13 mA,and the difference between them was statistically significant(P<0.05).Conclusion Before and after open round window membrane,the auditory function of healthy guinea pigs showed obvious changes,which showed hearing loss.

关 键 词：健康豚鼠 圆窗膜挑开 听性脑干反应 耳蜗电图 电诱发复合动作电位 电诱发听性脑干反应 

分 类 号：R764.05[医药卫生—耳鼻咽喉科]