大豆NRT2家族基因的鉴定、特征及表达模式  

Identification,Characterization and Expression Patterns of the NRT2 Gene Family in Soybean

作　　者：隋超 孙永旺 郭尚敬[1] Sui Chao;Sun Yongwang;Guo Shangjing(College of Agriculture,Liaocheng University,Liaocheng,252000)

机构地区：[1]聊城大学农学院,聊城252000

出　　处：《分子植物育种》2024年第13期4185-4191,共7页Molecular Plant Breeding

基　　金：山东省农业科技园区产业提升工程项目(2019YQ035)资助。

摘　　要：硝酸盐转运蛋白2 (Nitrate transporter 2, NRT2)是一种高亲和硝酸盐转运蛋白,在许多植物对硝酸盐的吸收和转运过程中发挥重要作用。本研究旨在对大豆NRT2基因家族进行全基因组鉴定和生物信息学分析。结果表明,大豆中有6个NRT2基因,分布在5条染色体上。根据进化关系可将大豆NRT2家族基因分为两类,每类中的基因结构和保守结构域分布相似。GmNRT2启动子区域均含有大量的光响应元件,这表明GmNRT2基因的表达可能受光信号调控。表达模式分析发现,大豆NRT2家族基因主要在根和根毛中表达,表明该家族基因与氮素吸收可能有着紧密的关系。本研究结果为大豆NRT2家族基因生物学功能研究提供了一定基础。Nitrate transporter 2(NRT2)is a high affinity nitrate transporter,which plays an important role in nitrate uptake and transport in many plants under nitrogen stress.In this study,we performed genome-wide identification and bioinformatics analysis of the NRT2 family genes in soybean genome.The results showed that there were six NRT2 family genes in soybean genome,which were distributed on five chromosomes.Phylogenetic analysis showed that soybean NRT2 family genes could be clustered into two groups,members in the same group had similar gene structure,and their conserved domains share similar distribution pattern.There were a large number of light response elements distributed in promoter regions of GmNRT2s,which indicates that the expression of GmNRT2 gene may be regulated by light signal.Expression pattern analysis showed that GmNRT2s were mainly expressed in root and root hairs,which indicates that GmNRT2s may be closely related to nitrogen uptake.These results provide a reference for further exploring of the biological function of the soybean NRT2 family genes.

关 键 词：大豆 NRT2基因家族 硝酸盐 氮吸收 

分 类 号：S565.1[农业科学—作物学]