地被菊CmbZIPs基因的生物信息及表达模式分析  

Analysis of Biological Information and Expression Pattern of CmbZIPs Gene in Ground-cover Chrysanthemum

作　　者：陈慧 杨雯婷 何子涵 张佳凝 张发赓 殷铭 王耀莹 朱佳琪 高日[2] Chen Hui;Yang Wenting;He Zihan;Zhang Jianing;Zhang Fageng;Yin Ming;Wang Yaoying;Zhu Jiaqi;Gao Ri(College of Agricultural Management,Jilin Open University,Changchun,130000;College of Agriculture,Yanbian University,Yanji,133002)

机构地区：[1]吉林开放大学农业管理学院,长春130000 [2]延边大学农学院,延吉133002

出　　处：《分子植物育种》2024年第13期4203-4211,共9页Molecular Plant Breeding

基　　金：国家自然科学基金项目(32060696);延边州科技发展计划项目(2020FG31);延边大学校企合作项目(延大科合字2020);延边大学博士科研启动基金项目(2018);吉林省大学生创新创业训练计划项目(S202110184133)共同资助。

摘　　要：地被菊(Chrysanthemum morifolium)为菊科菊属多年生草本花卉,其具有花朵繁密、花色丰富、抗逆性强等特点,是园林绿化美化的优良品种。土壤的盐渍化和低温严重限制地被菊的分布。植物中HD-ZIP基因家族广泛参与植物非生物胁迫。本研究以地被菊为材料,根据转录组数据克隆了CmbZIP12、CmbZIP24、CmbZIP10、CmbZIP3基因,对其氨基酸序列、保守结构域等进行了生物信息学分析。在低温胁迫和NaCl胁迫处理下,利用RT-qPCR分析了这些基因的表达特性。结果表明,CmbZIP12、CmbZIP24、CmbZIP10、CmbZIP3基因ORF在453~630 bp之间,CmbZIP12和CmbZIP3为酸性蛋白,CmbZIP24和CmbZIP10为碱性蛋白,CmbZIP12、CmbZIP10和CmbZIP3为不稳定蛋白,CmbZIP24为稳定蛋白;利用MEME软件预测CmZIPs基序组成,共鉴定出4个保守基序;系统发育分析显示,CmbZIP12和CmbZIP3为HD-ZIPⅠ亚家族,CmbZIP10为HD-ZIPⅡ亚家族,CmbZIP24为HD-ZIPⅢ亚家族;互作网络预测发现CmbZIP12与GBF3、SNRK2.3、OST1等蛋白存在网络互作关系;CmbZIP24与AT5G04840、ZIP2、BZIP34等蛋白互作。低温和盐处理地被菊幼苗发现,CmbZIP12、CmbZIP24、CmbZIP10、CmbZIP3相对表达量都明显增加。本研究可为地被菊CmbZIP12、CmbZIP24、CmbZIP10、CmbZIP3基因功能的研究提供参考。Ground-cover chrysanthemum(Chrysanthemum morifolium)is a perennial herbaceous flower of Dendranthema genus in the family Compositae,in which has the characteristics of dense flowers,rich flower colors and strong stress resistance,and is an excellent variety for landscaping.However,the distribution of ground cover-chrysanthemum is restricted by low temperature and soil salinization.The HD-ZIP family is widely involved in abiotic stress in plants.In this study,CmbZIP12,CmbZIP24,CmbZIP10,CmbZIP3 genes were cloned from ground-cover chrysanthemum based on transcriptome data,and its amino acid sequence and conserved domain were analyzed by bioinformatics.Under low temperature and NaCl abiotic stress,the expression characteristics of these genes were analyzed by RT-qPCR.The results showed that the ORF lengths of CmbZIP12,CmbZIP24,CmbZIP10,and CmbZIP3 genes were ranged from 453 to 630 bp.CmbZIP12 and CmbZIP3 were acidic proteins,and CmbZIP24 and CmbZIP10 were basic proteins;CmbZIP12,CmbZIP10 and CmbZIP3 were unstable proteins,and CmbZIP24 was a stable protein.Four conserved motifs were identified by MEME software.Phylogenetic analysis showed that CmbZIP12,CmbZIP3 belonged to HD-ZIPⅠsubfamily;CmbZIP10 and CmbZIP24 belong to HD-ZIPⅡand HD-ZIPⅢsubfamily,respectively.Interaction network prediction showed that CmbZIP12 had network interaction with GBF3,SNRK2.3,OST1 and other proteins;CmbZIP24 interacted with AT5G04840,ZIP2,BZIP34 and other proteins.The relative expression levels of CmbZIP12,CmbZIP24,CmbZIP10 and CmbZIP3 were significantly increased by low temperature and salt treatment.This study can provide reference for the functions of genes CmbZIP12,Cmb-ZIP24,CmbZIP10 and CmbZIP3 in ground-cover chrysanthemum.

关 键 词：地被菊 CmbZIP 蛋白互作 低温胁迫 盐胁迫 

分 类 号：S682.11[农业科学—观赏园艺]