甜瓜PP2C基因家族的鉴定和生物信息学分析  

Identification and Bioinformatics Analysis of PP2C Gene Family in Melon

作　　者：韩子超 张月洋 刘成功 李壑 苏婷婷 祝建波[1] Han Zichao;Zhang Yueyang;Liu Chenggong;Li He;Su Tingting;Zhu Jianbo(School of Life Sciences,Shihezi University,Shihezi,832003)

机构地区：[1]石河子大学生命科学学院,石河子832003

出　　处：《分子植物育种》2024年第13期4222-4232,共11页Molecular Plant Breeding

基　　金：兵团重点项目优质耐贮运哈密瓜品系的研究与生产示范(0155/KZ0118)资助。

摘　　要：PP2C是一类对生物体具有重要调控作用的蛋白磷酸酶。为研究其在甜瓜生长发育过程中的功能,本研究利用生物信息学方法对甜瓜中PP2C基因家族进行了分析。结果表明,在甜瓜基因组中共包含有61个PP2C基因家族成员,分为11个亚族。motif和基因结构分析结果显示在同一亚家族内,CmPP2C基因的基因结构与motif具有较高的相似性。基因复制事件分析结果表明,片段重复事件是CmPP2C基因家族扩张的主要原因,且CmPP2C基因成员在进化过程中可能经历了严格的纯化选择。表达分析结果显示CmPP2C基因在甜瓜不同组织内呈不均匀分布,有些基因表现出显著的组织特异性。此外,启动子分析结果表明在CmPP2C基因启动子序列中分布有多个植物激素和逆境相关的响应元件。本研究为甜瓜PP2C基因家族生物学功能的研究提供一定的参考。PP2C is a class of protein phosphatases that play a crucial regulatory role in organisms.To study their function in the growth and development of melon,this study analyzed the PP2C gene family in melon using bioinformatics methods.The results showed that there are a total of 61 PP2C gene family members in the melon genome,divided into 11 subfamilies.Motif and gene structure analyses revealed that CmPP2C genes within the same subfamily have high similarities in gene structure and motifs.Gene duplication event analysis indicated that segmental duplication is the primary reason for the expansion of the CmPP2C gene family,and CmPP2C gene members might have undergone strict purifying selection during evolution.Expression analysis showed that CmPP2C genes are unevenly distributed across different tissues of melon,with some genes exhibiting significant tissue specificity.Additionally,promoter analysis showed that multiple response elements related to plant hormones and stress are present in the promoter sequences of CmPP2C genes.This study provides some reference for the research on the biological functions of the melon PP2C gene family.

关 键 词：甜瓜 PP2C 基因家族 生物信息学 表达分析 

分 类 号：S652[农业科学—果树学] Q811.4[农业科学—园艺学]