七种石斛属植物花朵的挥发性成分分析  

Analysis of Volatile Components in the Flowers of Seven Species of Dendrobium

作　　者：吴君楠 刘子毓 武美卿 王海秋 皇晶晶 余文刚[1] Wu Junnan;Liu Ziyu;Wu Meiqing;Wang Haiqiu;Huang Jingjing;Yu Wengang(College of Horticulture,Hainan University,Haikou,570228)

机构地区：[1]海南大学园艺学院,海口570228

出　　处：《分子植物育种》2024年第13期4347-4356,共10页Molecular Plant Breeding

基　　金：国家自然科学基金项目(31660580);海南省省部重点实验室开放课题(RRI-KLOF202003)共同资助。

摘　　要：为了解7种石斛属植物花朵中的挥发性成分,采用固相微萃取(SPME)法和GC-MS技术,对石斛(Dendrobium nobile Lindl.)、肿节石斛(Dendrobium pendulum Roxb.)、大苞鞘石斛(Dendrobium wardianum Warner)、杯鞘石斛(Dendrobium gratiosissimum Rchb. F.)、报春石斛(Dendrobium primulinum Lindl.)、玫瑰石斛(Dendrobium crepidatum Lindl. ex Paxton)和流苏石斛(Dendrobium fimbriatum Hook.) 7种植物盛花期花朵的挥发性成分进行测定和分析。结果显示,从7种石斛花朵中共鉴定出挥发性成分77种,包括萜烯类、烷烃类、炔烃类、酯类、醛类、醇类、酮类和芳香族化合物等8类,7种石斛花中的挥发性成分既有相同的成分,也有不同的成分,随种类不同其主要挥发性成分和各成分含量也有差异。石斛以α-蒎烯(30.85%)为主,肿节石斛以β-石竹烯(45.18%)为主,大苞鞘石斛以罗勒烯(13.42%)和α-伊兰烯(13.32%)为主,杯鞘石斛β-硒烯(15.13%)为主,报春石斛以乙酸辛酯(70.53%)为主,玫瑰石斛以α-蒎烯(26.52%)和(-)-α-蒎烯(25.02%)为主,流苏石斛以α-硒烯(27.76%)为主。本研究结果可作为石斛兰香型品种的选育参考,也可作为资源开发利用的参考依据。In order to understand the volatile components in the flowers of 7 species of Dendrobium,using the solid-phase microextraction(SPME)method and the GC-MS technology,for Dendrobium nobile Lindl.,Dendrobium pendulum Roxb.,Dendrobium wardianum Warner,Dendrobium gratiosissimum Rchb.F.,Dendrobium primulinum Lindl.,Dendrobium crepidatum Lindl.ex Paxton and Dendrobium fimbriatum Hook.of the flowering flowers were determined and analyzed.The results showed that a total of 77 volatile components were identified from 7 species of Dendrobium flowers,including 8 types of terpenes,alkanes,alkynes,esters,aldehydes,alcohols,ketones and aromatic compounds.The volatile components in the seven species of Dendrobium were the same or different,and the main volatile components and the contents of each component were also different with different species.D.nobile mainly containedα-pinene(30.85%),D.pendulum mainly containedβ-caryophyllene(45.18%),D.wardianum mainly containedβ-ocimene(13.42%)andα-ylangene(13.32%),D.gratiosissimum mainly containedβ-selenene(15.13%),D.primulinum mainly contained octyl acetate(70.53%),D.crepidatum mainly containedα-pinene(26.52%)and(-)-α-pinene(25.02%),D.fimbriatum mainly containedα-selenene(27.76%).The results of this study can be used as a reference for the selection and breeding of aromatic varieties of Dendrobium orchids,and can also be used as a reference for resource development and utilization.

关 键 词：石斛属 花香成分 SPME GC-MS 

分 类 号：S682.31[农业科学—观赏园艺]