Diamond-Like Carbon Depositing on the Surface of Polylactide Membrane for Prevention of Adhesion Formation During Tendon Repair  

作　　者：Yao Xiao Zaijin Tao Yufeng Ju Xiaolu Huang Xinshu Zhang Xiaonan Liu Pavel A.Volotovski Chao Huang Hongqi Chen Yaozhong Zhang Shen Liu 

机构地区：[1]Department of Orthopaedics,Shanghai Jiao Tong University School of Medicine Affiliated Sixth People’s Hospital,600 Yishan Rd,Shanghai 200233,People’s Republic of China [2]Shanghai Tongji Hospital,389 Xincun Rd,Shanghai 200065,People’s Republic of China [3]Key Laboratory for Thin Film and Microfabrication of Ministry of Education,Research Institute of Micro/Nano Science and Technology,Shanghai Jiao Tong University,Shanghai 200240,People’s Republic of China [4]Orthopedic Trauma Department,Belarus Republic Scientific and Practical Center for Traumatology and Orthopedics,Kizhevatova str.,60/4,220024 Minsk,Belarus [5]Shanghai Haohai Biological Technology Limited Liability Company,1386 Hongqiao Rd,Shanghai 200336,People’s Republic of China [6]Department of General Surgery,Shanghai Jiao Tong University School of Medicine Affiliated Sixth People’s Hospital,600 Yishan Rd,Shanghai 200233,People’s Republic of China [7]Shanghai Key Laboratory for High Temperature Materials and Precision Forming,School of Materials Science and Engineering,Shanghai Jiao Tong University,Shanghai 200240,People’s Republic of China

出　　处：《Nano-Micro Letters》2024年第9期478-499,共22页纳微快报（英文版）

基　　金：supported by the National Natural Science Foundation of China(No.82172408,81772314,and 81922045);the Original Exploration project(22ZR1480300);Outstanding Academic Leaders(Youth)project(21XD1422900)of Shanghai Science and Technology Innovation Action Plan;Principle Investigator Innovation Team of Both Shanghai Sixth People’s Hospital and Shanghai Institute of Nutrition and Health,Shanghai Jiao Tong University Medical College“Two-hundred Talent”Program(No.20191829);The Second Three-Year Action Plan for Promoting Clinical Skills and Clinical Innovation in Municipal Hospitals of Shanghai Shenkang(No.SHDC2020CR4032);Shanghai Excellent Academic Leader Program;Shanghai Engineering Research Center for Orthopaedic Material Innovation and Tissue Regeneration(No.20DZ2254100);China Postdoctoral Science Foundation(2023M742347).

摘　　要：Post-traumatic peritendinous adhesion presents a significant challenge in clinical medicine.This study proposes the use of diamond-like carbon(DLC)deposited on polylactic acid(PLA)membranes as a biophysical mechanism for anti-adhesion barrier to encase ruptured tendons in tendon-injured rats.The results indicate that PLA/DLC composite membrane exhibits more efficient anti-adhesion effect than PLA membrane,with histological score decreasing from 3.12±0.27 to 2.20±0.22 and anti-adhesion effectiveness increasing from 21.61%to 44.72%.Mechanistically,the abundant C=O bond functional groups on the surface of DLC can reduce reactive oxygen species level effectively;thus,the phosphorylation of NF-κB and M1 polarization of macrophages are inhibited.Consequently,excessive inflammatory response augmented by M1 macrophage-originated cytokines including interleukin-6(IL-6),interleukin-1β(IL-1β),and tumor necrosis factor-α(TNF-α)is largely reduced.For biocompatibility evaluation,PLA/DLC membrane is slowly absorbed within tissue and displays prolonged barrier effects compared to traditional PLA membranes.Further studies show the DLC depositing decelerates the release of degradation product lactic acid and its induction of macrophage M2 polarization by interfering esterase and PLA ester bonds,which further delays the fibrosis process.It was found that the PLA/DLC membrane possess an efficient biophysical mechanism for treatment of peritendinous adhesion.

关 键 词：Diamond-like carbon Reactive oxygen species scavenging Foreign body reaction BIODEGRADATION ANTIOXIDANT Peritendinous adhesion 

分 类 号：R318.08[医药卫生—生物医学工程] TQ323.4[医药卫生—基础医学] TB306[化学工程—合成树脂塑料工业]