基于荧光方法的循环肿瘤细胞检测研究进展  

Research Progress of Circulating Tumor Cell Detection Based on Fluorescence Method

作　　者：杨瑞 李子越 申艺 郭丽华 周兵帅 李家玮 刘海鹏[2] 董彪 YANG Rui;LI Ziyue;SHEN Yi;GUO Lihua;ZHOU Bingshuai;LI Jiawei;LIU Haipeng;DONG Biao(State Key Laboratory of Integrated Optoelectronics,College of Electronic Science and Engineering,Jilin University,Changchun 130012,China;Department of Plastic and Aesthetic Surgery,The First Hospital of Jilin University,Changchun 130012,China)

机构地区：[1]吉林大学电子科学与工程学院,集成光电子学国家重点实验室,吉林长春130012 [2]吉林大学第一医院整形美容与修复重建外科,吉林长春130012

出　　处：《发光学报》2024年第6期1015-1032,共18页Chinese Journal of Luminescence

基　　金：国家自然科学基金(52050077,52272080,82073475);吉林省自然科学基金(20220402005GH)。

摘　　要：循环肿瘤细胞(Circulating tumor cells,CTCs)是指从恶性肿瘤的原发或转移部位脱落的细胞,通过血液循环到达全身。体内CTCs的存在可以反映肿瘤的发生与发展,对肿瘤的诊断和预后至关重要。然而,实现高纯度捕获和捕获后CTCs灭活阻断仍然面临许多挑战。目前开发的用于实现选择性分离CTCs的方案中,荧光方法由于具有高灵敏度、高分辨率、操作简便等特点,在无创检测和快速检测方面具有重要的应用前景。与以往的CTCs研究综述相比,本文详细介绍了CTCs从体外捕获到体内捕获再到下游分析的全过程,并对CTCs的完整诊疗过程进行了系统和详细的总结,为当前的研究提供了新思路,这对于实现早期循环肿瘤细胞的诊断与治疗具有较重要意义。Circulating tumor cells(CTCs)are cells that are shed from the primary or metastatic site of a malignant tumor and circulate through the bloodstream to reach the body.Therefore,the presence of CTCs in vivo can reflect the occurrence and development of tumors,which is crucial for the diagnosis and prognosis of tumors.However,there are still many challenges to achieving high purity capture and post-capture CTCs inactivation blocking.Among the schemes developed for selective separation of CTCs,fluorescence method has an important application prospect in non-invasive detection and rapid detection because of its high sensitivity,high resolution and simple operation.Compared with previous CTCs research reviews,this paper describes the whole process of CTCs from in vitro capture to in vivo capture to downstream analysis in detail,and systematically and detailedly summarizes the complete diagnosis and treatment process of CTCs,providing new ideas for current research.This is of great significance for the diagnosis and treatment of early circulating tumor cells.

关 键 词：循环肿瘤细胞 荧光 捕获 下游分析 灭活 

分 类 号：O657.3[理学—分析化学]