气相色谱-质谱法测定鱼油中EPA和DHA 含量的方法研究  

GC-MS Determination of DHA and EPA in Fish Oil Health Products

作　　者：康牧旭 赵旭峰 张晶晶 张晓威 侯文锐 武卫影 刘华梁 郭喜强 吕学冲 KANG Mu-xu;ZHAO Xu-feng;ZHANG Jing-jing;ZHANG Xiao-wei;HOU Wen-rui;WU Wei-ying;LIU Hua-liang;GUO Xi-qiang;LV Xue-chong(Xingtai Inspection and Testing Center,Hebei Xingtai 054000;Hengshui Comprehensive Inspection and Testing Center,Hebei Hengshui 053000;Center for Adverse Drug Reactions and Drug Abuse Monitoring of Xingtai,Hebei Xingtai 054000;Ningjin County Food and Drug Inspection and Testing Center,Hebei Xingtai 055550;Julu County Comprehensive Inspection and Testing Center,Hebei Xingtai 055250;Xingtai University,Hebei Xingtai 054000;Baoding Institute of Product Quality Inspection,Hebei Baoding 071000,China)

机构地区：[1]邢台市检验检测中心,河北邢台054000 [2]衡水市综合检验检测中心,河北衡水053000 [3]邢台市药品不良反应和药物滥用监测中心,河北邢台054000 [4]宁晋县食品药品检验检测中心,河北邢台055550 [5]巨鹿县综合检验检测中心,河北邢台055250 [6]邢台学院,河北邢台054000 [7]保定市产品质量监督检验所,河北保定071000

出　　处：《广州化工》2024年第6期124-127,共4页GuangZhou Chemical Industry

基　　金：河北省市场监督管理局科研计划项目(No:2022ZC45)。

摘　　要：建立气相色谱-质谱(GC-MS)法测定鱼油中EPA、DHA的含量。方法:采用安捷伦DB-FastFAME石英毛细管柱(20 m×0.18 mm×0.20μm),质谱检测器,内标法定量检测。样品经由氢氧化钠-甲醇溶液甲酯化,经离心过滤后由GC-MS法测定。结果:EPA和DHA分别在27.150~434.41μg/mL和27.244~435.91μg/mL质量浓度范围内与峰面积呈良好的线性关系(相关系数分别为0.995 9和0.997 5)。该方法EPA和DHA的检出限分别为1.530μg/g、2.312μg/g,样品平均添加回收率为96.92%~100.42%。A method for determination of DHA and EPA in fish oil health products was established by gas chromatography-mass spectrometry(GC-MS).DB-FastFAME capillary column(20 m×0.18 mm×1.8μm)and MS detector were employed.Internal standard was used for quantitative determination.The sample material was methylated with NaOH-methanol solution,centrifuged and filtrated.The final extract was analyzed by GC-MS.The results showed that mass concentrations and peak areas of EPA and DHA had good linear relationships in ranges of 27.150~434.41μg/mL and 27.244~435.91μg/mL.The method detection limit was 1.530μg/g for EPA,and 2.312μg/g for DHA.The average recoveries were 96.92%~100.42%.

关 键 词：气相色谱-质谱法 内标法 EPA DHA 

分 类 号：TS221[轻工技术与工程—粮食、油脂及植物蛋白工程] O657.1[轻工技术与工程—食品科学与工程]