电感耦合等离子发射光谱法与钒钼黄比色法在有效磷含量测定中的应用比较  

Comparison of inductively coupled plasma emission spectrometry and vanadium molybdenum yellow colorimetric methods on available phosphorus determination

作　　者：华丽霞[1,2] 代顺冬 何炼[1,2] 叶鹏盛[1,2] 孙小芳 况再银[1] 曾华兰 蒋秋平 张敏 HUA Li-xia;DAI Shun-dong;HE Lian;YE Peng-sheng;SUN Xiao-fang;KUANG Zai-yin;ZENG Hua-lan;JIANG Qiu-ping;ZHANG Min(Industrial Crop Research Institute,Sichuan Academy of Agricultural Sciences,Chengdu Sichuan 610300;Key Laboratory of Integrated Pest Management on Crops in Southwest,Ministry of Agriculture and Rural Affairs,Chengdu Sichuan 610300)

机构地区：[1]四川省农业科学院经济作物研究所,四川成都610300 [2]农业农村部西南作物有害生物综合治理重点实验室,四川成都610300

出　　处：《中国土壤与肥料》2024年第4期247-252,共6页Soil and Fertilizer Sciences in China

基　　金：四川省科技厅自然科学基金项目(2023NSFSC1261);财政部和农业农村部国家现代农业产业技术体系(CARS-21);现代农业产业技术体系四川豆类杂粮创新团队(SCCXTD-2020-20);四川省财政自主创新项目(2022ZZCX081,2024ZZCX007);四川省农业科学院拔尖人才研究基金(2020BJRC023)。

摘　　要：在农业生产过程中需要对土壤中的有效磷含量以及肥料中的有效磷含量进行检测,为配方施肥提供重要依据,有效磷检测方法的准确性是评判肥料质量高低、实现配方施肥的关键。研究对电感耦合等离子发射光谱法与钒钼黄比色法这两种有效磷检测方法的灵敏度和准确度进行了比较,明确了电感耦合等离子发射光谱法在213.6 nm条件下的磷检出限为0.05 mg/L,钒钼黄比色法在400 nm检测波长的灵敏度最高,检出限为0.32 mg/L。对两种检测方法的检测准确度进行比较,结果表明电感耦合等离子发射光谱法对不同浓度的有效磷检测准确度均高于96%,其准确度受磷浓度的影响小;传统的钒钼黄比色法检测准确度受磷浓度、检测波长影响较大,对磷含量低于5 mg/L的样品检测准确度下降,且低于电感耦合等离子发射光谱法的检测准确度。研究结果认为对于未知浓度范围的样品,钒钼黄比色法在400 nm波长检测条件下的检测结果较为理想。In the process of agricultural production,available phosphorus determination of cropland soil and fertilizer is necessary for formula fertilization protocol establishment.The accuracy of detection methods is important for judging the quality of fertilizer and realizing formula fertilization.In this study,the sensitivity and accuracy of inductively coupled plasma emission spectrometry(ICP-OES)and vanadium molybdenum yellow colorimetric methods were compared on available phosphorus determination,it was found that the determination limit of ICP-OES method was 0.05 mg/L at 213.6nm wavelength,while it was 0.32 mg/L for the vanadium molybdenum yellow colorimetric method at 400 nm.The detection accuracy of the two methods was compared.It showed that the accuracy of ICP-OES method was not affected by phosphorus concentration as the determination accuracy of different phosphorus concentration standard samples was higher than 96%.However,the accuracy of traditional vanadium molybdenum yellow colorimetric method was greatly affected by phosphorus concentration and detection wavelength,as detection accuracy was greatly reduced for samples with phosphorus concentration less than 5 mg/L,and the accuracy was lower than that of ICP-OES method.Based on the results,400 nm detection wavelength was a ideal condition for vanadium molybdenum yellow colorimetric method for phosphorus determination of unknown samples.

关 键 词：有效磷 电感耦合等离子体发射光谱法 钒钼黄比色法 肥料 

分 类 号：S153.6[农业科学—土壤学]