瑞香狼毒提取物对3种蔬菜枯萎病病原真菌的抑制作用  

Antimicrobial Activity of Extracts from Stellera chamaejasme Against Three Vegetable Pathogenic Fungi

作　　者：孙婧 成立新 李蕴华 岳林芳 刘芳 庞亚娟 SUN Jing;CHENG Lixin;LI Yunhua;YUE Linfang;LIU Fang;PANG Yajuan(College of Horticulture and Plant Protection,Inner Mongolia Agricultural University,Hohhot 010018;Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences,Hohhot 010031;College of Science,Inner Mongolia Agricultural University,Hohhot 010018;Erdos Agricultural and Animal Husbandry Technology Extension Center,Erdos,Inner Mongolia 017010)

机构地区：[1]内蒙古农业大学园艺与植物保护学院,呼和浩特010018 [2]内蒙古自治区农牧业科学院,呼和浩特010031 [3]内蒙古农业大学理学院,呼和浩特010018 [4]鄂尔多斯市农牧技术推广中心,内蒙古鄂尔多斯017010

出　　处：《中国农学通报》2024年第19期115-119,共5页Chinese Agricultural Science Bulletin

基　　金：内蒙古自治区高等学校科学研究项目“瑞香狼毒与有机肥配施对设施蔬菜立枯病的防效及其促生作用研究”(NJZY21475);内蒙古自治区科技计划项目“畜禽养殖有机废弃物资源化利用模式研究与示范”(2020GG0040)。

摘　　要：为开发可抑制设施蔬菜类病原菌的植物源杀菌剂,设置不同浓度的瑞香狼毒无水乙醇提取物,采用菌丝生长速率法分别研究其对立枯丝核菌、尖孢镰刀菌和变黑轮枝菌活性的抑制情况,并进行毒力分析。结果表明,瑞香狼毒提取物对3种病原菌均有抑制作用,且抑制作用随提取液浓度增大而增强;当提取液浓度分别为8、10mg/mL时,对立枯丝核菌、尖孢镰刀菌的抑制率均能够达到50%以上,且当提取液浓度为15mg/mL时,抑菌率值最大,分别为87.38%和92.84%;而当提取液浓度达到20mg/mL时其对变黑轮枝菌的抑制率才能达到50%以上(59.09%)。有效中浓度EC50值由大到小为变黑轮枝菌(15.64 mg/mL)>尖孢镰刀菌(9.08 mg/mL)>立枯丝核菌(5.62 mg/mL)。瑞香狼毒提取物对供试3种病原真菌均具有抑制作用,其中对立枯丝核菌的抑制作用最强,对变黑轮枝菌抑制作用最弱。To develop botanical fungicide that can inhibit vegetable pathogens in protected cultivation,anhydrous ethanol extracts of Stellera chamaejasme with different concentration gradients were used to study the inhibitory activity of Rhizoctonia solani,Fusarium oxysporum and Gibellulopsis nigrescens by mycelial growth rate method,and the virulence was analyzed.The results showed that different concentrations of S.chamaejasme extracts had different degrees of inhibitory effect on three vegetable pathogens,and the inhibitory effect was enhanced with the increase of extract concentration.When the concentration of the extract were 8 mg/mL and 10 mg/mL,the inhibition rate of R.solani and F.oxysporum could reach more than 50%,and when the concentration of the extract was 15 mg/mL,the inhibition rate was the highest,which was 87.38%and 92.84%,respectively.Only when the concentration of the extract reached 20 mg/mL,the inhibition rate of the extract against G.nigrescens could reach over 50%(59.09%).The EC50 values of effective medium concentration were as follows:G.nigrescens(15.64 mg/mL)>F.oxysporum(9.08 mg/mL)>R.solani(5.62 mg/mL).Therefore,the good antibacterial effect of extracts of S.chamaejasme is proven on all the three pathogenic fungi tested,among which the inhibitory effect on R.solani is the strongest,and that on G.nigrescens is the weakest.

关 键 词：瑞香狼毒 设施蔬菜 植物提取物 枯萎病 抑菌作用 

分 类 号：S482.292[农业科学—农药学]