聚合物胶束体内命运分析技术的研究进展  

Advances of Analytical Technologies for Studying in Vivo Fate of Polymeric Micelles

作　　者：于璐瑶 刘美辰[1] 郭莹霞 尤建嵩 史美云[1] 尹磊 YU Lu-yao;LIU Mei-chen;GUO Ying-xia;YOU Jian-song;SHI Mei-yun;YIN Lei(School of Chemical Engineering,Ocean and Life Sciences,Dalian University of Technology,Panjin 124221,China;Aim Honesty Biopharmaceutical Co.,Ltd.,Dalian 116600,China)

机构地区：[1]大连理工大学化工海洋与生命学院,辽宁盘锦124221 [2]艾美诚信生物制药有限公司,辽宁大连116600

出　　处：《化学试剂》2024年第7期75-84,共10页Chemical Reagents

基　　金：国家自然科学基金项目(81603182,81703607);中央高校基本科研业务费专项资金项目(DUT21RC(3)057,DUT23YG228,DUT24MS018);大连市科技创新基金项目(2022JJ13SN075);辽宁省自然科学基金项目(2023-MSBA-018);辽宁省肿瘤医院“肿瘤+”项目(2024-ZLKF-33)。

摘　　要：聚合物胶束(PMs)是研究非常广泛的纳米给药系统。它是由两亲性聚合物组成的自聚集纳米颗粒,这些聚合物在临界胶束浓度(CMC)以下以单分子形式存在于溶液中,在CMC以上则会自组装形成胶束。相对于其他纳米载体,PMs具有独特的性质:相对较小的尺寸,疏水性核对疏水性化合物具有良好溶解性,亲水性壳能延长药物在血液的循环时间。对PMs体内时空命运的未知,使得PMs从实验室设计到临床应用的成功转化仍然面临重大挑战。目前,一系列分析测试技术已经开发出来用于揭示PMs的体内命运,包括放射性示踪,荧光共振能量转移(FRET)、聚集诱导发射(AIE)、聚集荧光猝灭(ACQ)、液质联用(LC-MS),透射电子显微镜(TEM)等。主要叙述了一系列分析技术在PMs体内命运研究中的进展。Polymer micelles(PMs)are extensively studied nano delivery systems consisting of amphiphilic polymers that form self-aggregating nanoparticles.These nanoparticles exist as individual molecules in solution below a critical micelle concentration(CMC)but self-assemble into micelles above this threshold.PMs possess numerous advantages just like relatively small size,hydrophobic cores that promote the solubility of hydrophobic compounds,and hydrophilic shells that enhance drug delivery in the bloodstream.However,the transition of PMs from laboratory development to clinical application is challenged by the unknown spatiotemporal fate of PMs in vivo.To address this challenge,several analytical assays have been developed to elucidate the in vivo fate of PMs such as radiotracking,fluorescence resonance energy transfer(FRET),aggregation-induced emission(AIE),aggregation fluorescence burst(ACQ),liquid chromatography-mass spectrometry(LC-MS),transmission electron microscopy(TEM)and other analytical techniques.This article outlined the progress of these analytical techniques in the studying the in vivo fate of PMs.

关 键 词：聚合物胶束 体内命运 荧光生物成像 分析技术 研究进展 

分 类 号：O65[理学—分析化学]