肾脏类器官的研究及应用  

Research and application of kidney organoids

作　　者：王钢 王谊晴 梁菊(综述) 刘志红(审校) WANG Gang;WANG Yiqing;LIANG Ju;LIU Zhihong(National Clinical Research Center for Kidney Diseases,Jinling Hospital,Nanjing 210016,China)

机构地区：[1]东部战区总医院,国家肾脏疾病临床医学研究中心,南京210016 [2]浙江大学医学院

出　　处：《肾脏病与透析肾移植杂志》2024年第3期245-253,共9页Chinese Journal of Nephrology,Dialysis & Transplantation

基　　金：国家自然科学基金(32141004,82300800);国家重点研发计划(2021YFC2501302);江苏省自然科学基金(BK20210150)。

摘　　要：肾脏类器官领域的快速发展,为人类肾脏发育及肾脏疾病的研究提供了重要模型支持。肾脏类器官研究已从早期建立肾单位类器官为主,发展到输尿管胚芽/集合管类器官,从而可以更全面地模拟人类肾脏,使其更好地应用于肾脏发育模拟,疾病模型及药物筛选领域。此外,单细胞测序、多组学联用、基因编辑、器官芯片等新技术的交叉融合,使得对肾脏类器官的认识更全面和深入。本文将简介肾单位类器官以及输尿管胚芽/集合管类器官诱导方法、肾脏类器官细胞及结构鉴定优化、肾脏类器官的相关应用及改进措施。The rapid development of the field of kidney organoids has provided important model support for the study of human kidney development and kidney diseases.The research on kidney organoids has evolved from early establishment of nephron organoids to ureteric bud/collecting duct organoids,which can more comprehensively simulate human kidneys and better apply them in the fields of kidney development simulation,disease modeling,and drug screening.In addition,the cross fusion of new technologies such as single-cell sequencing,multi omics combination,gene editing,and organ-chips has also enabled researchers to have a more comprehensive and in-depth understanding of kidney organoids.This article will introduce the induction methods for nephron organoids and ureteric bud/collecting duct organoids,optimization of cell and structure for kidney organoids,relevant applications and improvement measures for kidney organoids.

关 键 词：肾脏类器官 诱导方法 疾病模型 

分 类 号：R692[医药卫生—泌尿科学]