基于生物信息学方法和动物实验方法获取多房棘球蚴病组蛋白H3K4ME1的生物学功能和信号通路信息  

Obtaining biological function and signaling pathway information of Alveolar echinococcosis histone H3K4ME1 based on bioinformatics and animal experimental methods

作　　者：朱鑫琳 陈佳昕 任利[1,2] ZHU Xinlin;CHEN Jiaxin;REN Li(Department of Hepatobiliary and Pancreatic Surgery,Qinghai University Affiliated Hospital;Qinghai Provincial Key Laboratory of Baoding Disease Research)

机构地区：[1]青海大学附属医院肝胆胰外科 [2]青海省包虫病研究重点实验室

出　　处：《中国高原医学与生物学杂志》2024年第2期124-130,共7页Journal of Chinese High Altitude Medicine & Biology

基　　金：青海省科技厅基础研究项目(2019-ZJ-7031)。

摘　　要：目的基于生物信息学方法和动物实验方法获取多房棘球蚴病组蛋白H3K4ME1的生物学功能和信号通路信息。方法1.提取冻存组织细胞核。2.构建cut&tag文库。3.分析生物信息:1)评估多房棘球蚴病组蛋白H3K4ME1的测序数据;2)确定reads在基因组上的分布;3)分析单个样本富集区间信息;4)分析peak转录因子;5)分析与Peak关联基因的GO功能富集、KEGG通路富集情况;6)分析差异关联基因基本情况;7)分析差异关联基因的GO功能富集情况;8)分析差异关联基因的KEGG通路富集情况。结果1.提取冻存组织细胞核:在显微镜下观察到细胞核计数>100000,表示细胞核提取成功。2.测序文库构建后,用qubit软件检查测序文库,结果显示合格。3.生物信息分析结果:1)实验组与参考基因库之间的数据相似程度>98%(测序数据良好),可进行下一步检测;2)多房棘球蚴病组和健康小鼠组的reads在TSS明显起峰;3)通过单个样本的富集,筛选出显著性Peak;4)与peak关联的转录因子主要集中在zf-C2H2、Homeobox和BHLH等;5)与Peak关联基因的GO功能主要富集在细胞蛋白大分子定位、细胞发育调节、解剖结构形态调控等生物学过程,与peak关联基因的KEGG通路主要富集在MAPK、cGMP-PKG、Hippo等信号通路;6)多房棘球蚴病组与健康小鼠组的组蛋白H3K4ME1存在5547个差异关联基因,其中上调基因2556个、下调基因2929个;7)组蛋白H3K4ME1差异关联基因GO功能富集分析的生物过程主要有细胞投射组织的正向调节、GTPase的活性调节等,细胞组分主要有细胞投影膜、突触等,分子功能主要有肌动蛋白结合、细胞黏附分子结合等;8)组蛋白H3K4ME1差异关联基因KEGG通路富集分析主要分布在MAPK、Wnt、Rap1等信号通路上。结论获得了多房棘球蚴病组蛋白H3K4ME1的生物学功能和信号通路信息。Objective To obtain the biological function and signaling pathway information of Alveolar echinococcosis histone H3K4ME1 based on bioinformatics methods and animal experimental methods.Methods 1.Extract the nuclei of frozen tissue.2.Build a cut&tag library.3.Analyze biological information:1)Evaluate the sequencing data of alveolar echinococcosis histone H3K4ME1;2)Determine the distribution of reads on the genome;3)Analyze the enriched interval information of a single sample;4)Analyze peak transcription factors;5)Analyze the GO functional enrichment and KEGG pathway enrichment of genes associated with Peak;6)Analyze the basic situation of differentially associated genes;7)Analyze the GO functional enrichment of differentially associated genes;8)Analyze the enrichment of differentially associated genes in the KEGG pathway.Results 1.The extraction of nuclei from frozen tissue: the nuclei count was >100 000 under the microscope, indicating successful extraction of the cell nuclei.2.After the sequencing library was constructed,the software qubit was used to check the sequencing library,and the results showed that it was qualified.3.Results of information analysis:1)The similarity between the experimental group and the reference genome was >98%(the sequencing data was good)for further testing;2)The reads of the alveolar echinococcosis group and the uninfected normal mice group showed significant peaks in TSS;3)Significant Peaks were identified through the enrichment screening of individual samples;4)Peak-associated transcription factors were mainly concentrated in zf-C2H2,Homeobox,and BHLH,etc.;5)The enrichment of the GO function of Peak-associated genes was mainly in the biological processes such as the localization of cellular proteins and macromolecules,cellular developmental regulation, and the regulation of anatomical structure and morphology. The KEGG pathway enrichment of Peak-associated genes was mainly enriched in MAPK,cGMP-PKG,Hippo and other signaling pathways;6)There were 5 547 differentially associated gen

关 键 词：多房棘球蚴病 组蛋白H3K4ME1 生物学 信号通路 

分 类 号：R383[医药卫生—医学寄生虫学]