基于非酒精性脂肪肝细胞模型的荷叶水提物降脂作用机制研究  

Study on Lipid-lowering Effects and Mechanisms of Lotus Leaf Water Extract Using an in vitro Model of Nonalcoholic Fatty Liver Disease

作　　者：洪祝平 张建华 史月姣 郑化仙 李振宇 徐靖 王瑛 杨继鸿 李振皓 HONG Zhuping;ZHANG Jjanhua;SHI Yuejiao;HENG Huaxian;LI Zhenyu;XU Jing;WANG Ying;YANG Jihong;LI Zhenhao(Zhejiang ShouXianGu Botanical Drug Institute Co.,Ltd.,Hangzhou 311121,China;Zhejiang ShouXianGu Pharmaceutical Co.,Ltd.,Jinhua 321200,China;Zhejiang Engineering Research Center of Rare Medicinal Plant,Jinhua 321200,China)

机构地区：[1]浙江寿仙谷植物药研究院有限公司,浙江杭州311121 [2]浙江寿仙谷医药股份有限公司,浙江金华321200 [3]浙江省珍稀植物药工程技术研究中心,浙江金华321200

出　　处：《现代中药研究与实践》2024年第2期41-47,共7页Research and Practice on Chinese Medicines

基　　金：浙江省重点研发计划(2017C02004);寿仙谷珍稀药材产品省级重点农业企业研究院(2017Y20001)。

摘　　要：目的研究荷叶水提物、荷叶碱、原荷叶碱及脱氢荷叶碱对脂代谢通路AMPK/SREBP1/ACC1的影响。方法采用超高效液相色谱-串联四级杆飞行时间质谱(UPLC-QTOF/MS)对荷叶水提物进行化学成分分析,并用FFA诱导的非酒精性脂肪肝HepG2细胞模型和BODIPY染色进行胞内脂质蓄积情况检测,用Western Blotting分析AMPK、SREBP1及ACC1等蛋白的表达水平。结果从荷叶水提物中共鉴定出15种化合物,主要为生物碱类和黄酮类化合物。FFA诱导后,荷叶碱、原荷叶碱均能降低HepG2细胞内脂质含量,下调升脂蛋白SREBP1及p-ACC1,并上调降脂蛋白p-AMPK的表达水平。此外,荷叶碱、原荷叶碱与荷叶水提物联合可增强降脂效果。结论荷叶碱及原荷叶碱可能为荷叶水提物中的主要降脂成分,其作用机制与调控AMPK/SREBP1/ACC1通路有关。Objective To investigate the effect of Lotus leaf water extract(LLWE),nuciferine(NF),pronuciferine(PF)and dehydronuciferine(DF)on lipid metabolism pathway AMPK-SREBP1-ACC1.Methods The UPLC-QTOF/MS was used to identify chemical constituents of LLWE.The HepG2 cellular model of NAFLD was induced by free fatty acids.The lipid accumulation was detected by BODIPY staining,and the Western Blotting determined the protein expression levels of AMPK,SREBP1 and ACC1.Results A total of 15 compounds were identified from LLWE by UPLC-QTOF/MS,mainly including alkaloids and flavonoids.The intervention of either NF or PF significantly reduced lipid accumulation with SREBP1 and p-ACC1 decreased,but increased p-AMPK in FFA-induced HepG2 cells.Additionally,the combination of either NF or PF with LLWE significantly enhanced the lipid-lowering effects of NF or PF alone.Conclusion NF and PF are main lipid-lowering cehmicals in LLWE,which might act through regulating the AMPK-SREBP1-ACC1 pathway.

关 键 词：荷叶水提物 荷叶碱 原荷叶碱 非酒精性脂肪肝 AMPK/SREBP1/ACC1通路 

分 类 号：R285.5[医药卫生—中药学]