亚精胺+赤霉素在低温下对化橘红种子萌发及幼苗生理指标的影响  

Effects of Spermidine+Gibberellin on Seed Germination and Seedling Physiological Indexes of Citri grandis Tomentosa at Low Temperatures

作　　者：冯世鑫[1] 陈乾平[1] 蒋妮[1] 宋利沙 FENG Shixin;CHEN Qianping;JIANG Ni;SONG Lisha(Medicinal Botanical Garden,Guangxi Zhuang Autonomous Region,Nanning,Guangxi 530023,China)

机构地区：[1]广西壮族自治区药用植物园,广西南宁530023

出　　处：《热带农业科学》2024年第4期43-47,共5页Chinese Journal of Tropical Agriculture

基　　金：广西重点研发计划(No.桂科AB20159014);广西中药材品质创新研究团队(No.GZKJ2305);广西道地药材高品质形成与应用重点实验室(No.ZJC2020003)。

摘　　要：以化橘红种子为材料,在低温下,研究亚精胺(Spd)+赤霉素(GA3)对化橘红种子萌发、幼苗根系的生长、根活力及生理指标的影响,以培育壮苗。设置亚精胺浓度为0(CK)、0.10、0.15、0.20、0.25 mmol/L,分别添加80 mg/L的GA3溶液浸种、培养,测定种子发芽率、发芽势,幼苗根长、苗长、根重、苗重、根长/苗长、根重/苗重、抗氧化酶活性、渗透调节物质及膜质过氧化物含量的变化。结果表明:在低温条件下,亚精胺+赤霉素对化橘红种子的萌发有协同促进作用,发芽率、发芽势和发芽指数明显提高;单一使用赤霉素时,幼苗根的生长受到抑制,添加亚精胺后能明显缓解,缓解程度与Spd的浓度呈正相关。0.20 mmol/L亚精胺+80 mg/L赤霉素处理的根长、根重、根长/苗长、根重/苗重和根活力最高,其幼苗根系的抗氧化酶(SOD、POD、CAT)活性显著增强,渗透调节物质脯氨酸和可溶性蛋白的含量有所提高,丙二醛(MDA)含量降低。亚精胺+赤霉素复配能促进化橘红种子萌发、根系的生长和根活力的提高;能缓解低温对化橘红幼苗根的损害,提高种苗的抗寒性。The effects of spermidine(Spd)+gibberellin(GA3)on seed germination,seedling root growth,root activity,and physiological indexes of Citri grandis Tomentosa were studied at low temperatures to cultivate sturdy seedlings.The concentrations of spermidine were set to 0(CK),0.10,0.15,0.20,and 0.25 mmol·L^(-1),respectively,and 80 mg·L^(-1) GA3 solution was added for soaking and culture.The germination rate,germination potential,seedling root length,seedling length,root weight,root length/seedling length,root weight/seedling weight,antioxidant enzyme activity,osmotic adjustment substance,and membrane peroxide content were measured.The results showed spermidine+gibberellic acid had a synergistic effect on the germination of C.grandis Tomentosa seeds,and the germination rate,germination potential,and germination index significantly improved at the condition of low temperature.When gibberellic acid was used alone,the growth of seedling roots was inhibited,and the addition of spermidine significantly alleviated this effect.The degree of alleviation was positively correlated with the concentration of Spd.The root length,root weight,root length/seedling length,root weight/seedling weight,and root activity were greatest in the treatment with 0.20 mmol·L^(-1) spermidine+80 mg·L^(-1) gibberellic acid.The activities of antioxidant enzymes(SOD,POD,and CAT)in the roots of the plants significantly increased.The contents of the osmotic adjustment substances proline and soluble protein increased,and the content of malondialdehyde(MDA)decreased.The combination of spermidine and gibberellic acid promoted the seed germination,root growth,and root activity of C.grandis Tomentosa;alleviated the damage caused by low temperature to the roots of C.grandis Tomentosa seedlings;and improved the cold resistance of the seedlings.

关 键 词：亚精胺 赤霉素 化橘红幼苗 根生长 生理指标 

分 类 号：R284.1[医药卫生—中药学]