一种优化的桑树花芽石蜡切片制作方法  

An Improved Method for Paraffin Sections of Mulberry Flower Buds

作　　者：李叶[1,2,3] 刘家粮 武华周 林培群 皇晶晶[1,4] 王树昌[1,2,3] LI Ye;LIU Jialiang;WU Huazhou;LIN Peiqun;HUANG Jingjing;WANG Shuchang(Environment and Plant Protection Institute,Chinese Academy of Tropical Agricultural Sciences,Haikou,Hainan 571101,China;Hainan Key Laboratory for Monitoring and Control of Tropical Agricultural Pests,Haikou,Hainan 571101,China;Key Laboratory of Integrated Pest Management on Tropical Crops,Ministry of Agriculture and Rural Affairs,P.R.China,Haikou,Hainan 571101,China;Hainan University,Haikou,Hainan 570228,China)

机构地区：[1]中国热带农业科学院环境与植物保护研究所,海南海口571101 [2]海南省热带农业有害生物监测与控制重点实验室,海南海口571101 [3]农业农村部热带作物有害生物综合治理重点实验室,海南海口571101 [4]海南大学,海南海口570228

出　　处：《热带农业科学》2024年第4期58-64,共7页Chinese Journal of Tropical Agriculture

基　　金：海南省自然科学基金资助(No.321MS072,No.321QN293)。

摘　　要：针对常规石蜡切片方法无法获得完整桑树花芽切片的问题,以“无籽大十”品种桑树花芽为供试材料,在常规石蜡切片技术基础上,对石蜡切片样品制作过程中固定、软化、脱水、透明及染色等环节进行了优化。结果表明,使用15%氢氟酸软化后,采用优化后的系列脱水、透明等步骤进行制片,可以使切片连续性、完整性得到明显提高。通过切片显微观察,可见细胞轮廓明显、裂痕较少、结构较完整,染色较清晰,并且对发育后期的花芽效果更为明显。优化后的制样流程解决了常规制片出现浸蜡不彻底、组织变脆、蜡带不连续、内部结构不完整等一系列问题。该研究可以从解剖学上为桑树花芽发育形态和花芽分化机制研究提供技术参考。To address the inability of the conventional paraffin sectioning method to obtain complete sections of mulberry flower buds,this study used“seedless 10”mulberry flower buds as test materials.Based on the conventional paraffin section technique,we optimized the fixation,softening,dehydration,transparency,and dyeing of paraffin section samples.The results show that after softening with 15%hydrofluoric acid,the continuity and integrity of the slices can be improved by adopting the optimized series of dehydration and transparency steps.Through microscopic observation,it can be seen that the cell outline is distinct,the cracks are few,the structure is complete,the staining is clear,and the effect on flower buds in the later stage of development is more distinct.The optimized sample preparation process has solved problems such as incomplete wax soaking,brittle structures,discontinuous wax bands,and incomplete internal structures in conventional production.This study provides a technical reference for the study of mulberry flower bud development morphology and flower bud differentiation mechanisms from an anatomical perspective.

关 键 词：桑树 花芽 石蜡切片 显微结构 

分 类 号：S888.1[农业科学—特种经济动物饲养]