血清Wnt4基因启动子区的DNA甲基化程度对早产患者预测价值分析  

Predictive value of DNA methylation degree of serum Wnt4 gene promoter region in preterm patients

作　　者：邱兰[1] 熊琼英 蔡大芬 QIU Lan;XIONG Qiongying;CAI Dafen(Department of Obstetrics and Gynecology,Xiaogan Hospital Affiliated to Wuhan University of Science and Technology,Xiaogan,Hubei 432000,China)

机构地区：[1]武汉科技大学附属孝感医院妇产科,湖北孝感432000

出　　处：《国际检验医学杂志》2024年第13期1580-1585,共6页International Journal of Laboratory Medicine

基　　金：湖北省孝感市科技计划科研项目(XGKJ2019010022)。

摘　　要：目的 分析血清Wnt4基因启动子区的DNA甲基化程度对早产患者的预测价值。方法 随机选择2019年1-12月在该院产科病区产检的孕产妇480例,其中450例随访至分娩,将其中40例自发性早产的孕产妇作为早产组,410例足月产孕产妇作为健康组。比较健康组、早产组、早产组不同孕周者的血清Wnt4基因启动子区的DNA甲基化水平。分析健康组、早产组的临床数据,采用Logistic回归分析早产的风险因素,绘制受试者工作特征(ROC)曲线分析血清Wnt4基因启动子区的DNA甲基化程度对早产的预测价值。结果 与健康组比较,早产组血清Wnt4基因启动子区的DNA甲基化程度明显增高(P<0.05);与早产组34~<37周妊娠期女性相比,孕周28~<34周妊娠期女性血清Wnt4基因启动子区的DNA甲基化程度明显增高(P<0.05);与健康组比较,早产组产次≥2次、妊娠期被动吸烟、阴道胎儿纤维连接蛋白(fFN)阳性、家庭平均月收入<1 000元的比例明显增加(P<0.05),宫颈长度明显缩短(P<0.05);Logistic回归分析结果显示,产次≥2次、妊娠期被动吸烟、宫颈长度短、阴道fFN阳性、家庭平均月收入<1 000元、血清Wnt4基因启动子区的DNA甲基化程度高均是早产的主要风险因素(P<0.05);ROC曲线分析结果表明,血清Wnt4基因启动子区的DNA甲基化程度预测早产的曲线下面积为0.868(95%CI:0.834~0.898),最佳截断值为51.43%,灵敏度、特异度、准确度分别为82.50%、84.63%、84.44%。结论 血清Wnt4基因启动子区的DNA甲基化程度高对于早产具有一定的临床预测价值。Objective To analyze the predictive value of DNA methylation degree of serum Wnt4 gene promoter region in preterm patients.Methods A total of 480 pregnant women were randomly selected from the obstetrics ward of the hospital from January to December 2019.Among them,450 were followed up until delivery,and 40 spontaneous preterm pregnant women were selected as the preterm group.410 full-term pregnant women were selected as the health group.The DNA methylation degree of serum Wnt4 gene promoter region was compared between health group and preterm group,and the pregnant women with different gestational week in preterm group.The clinical data of health group and preterm group were compared.Logistic regression analysis was used to analyze the influencing factors of premature delivery.Receiver operating characteristic(ROC)curve was plotted to analyze the predictive value of DNA methylation degree of serum Wnt4 gene promoter region for premature delivery.Results Compared with health group,the DNA methylation degree of serum Wnt4 gene promoter region in preterm group was significantly increased(P<0.05).Compared with pregnant women with 34-<37 gestational weeks in the preterm group,the DNA methylation degree of serum Wnt4 gene promoter region in pregnant women with 28-<34 gestational weeks was significantly increased(P<0.05).Compared with the health group,the proportions of pregnancies≥2 times,passive smoking during pregnancy,positive vaginal fetal fibronectin(fFN),and average monthly household income<1000 yuan in the preterm group were significantly increased(P<0.05),and cervical length was significantly shortened(P<0.05).Logistic regression analysis showed that pregnancies≥2 times,passive smoking during pregnancy,short cervical length,positive vaginal fFN,average monthly household income<1000 yuan and high degree of DNA methylation in serum Wnt4 gene promoter region were all risk factors for premature delivery(P<0.05).ROC curve results showed that the area under the curve of DNA methylation degree of serum Wnt4 gene

关 键 词：Wnt4基因 DNA甲基化程度 早产 预测 

分 类 号：R714.21[医药卫生—妇产科学]