种植抗异丙隆转基因水稻和施用异丙隆对转基因水稻根际微生物群落的影响  

Effects of planting isoproturon-resistant transgenic rice(Oryza sativa)and applying isoproturon on rhizospheric microbial communities of transgenic rice

作　　者：苏湘宁 李传瑛[1] 刘雪松 章玉苹[1] SU Xiang-ning;LI Chuan-ying;LIU Xue-song;ZHANG Yu-ping(Research Institute of Plant Protection,Guangdong Academy of Agricultural Sciences/Key Laboratory of Green Prevention and Control of Fruits and Vegetables in South China,Ministry of Agriculture and Rural Affairs/Key Laboratory of High Technology for Plant Protection of Guangdong Province,Guangzhou,Guangdong 510640,China;Institute of Agricultural Facilities and Equipment,Jiangsu Academy of Agricultural Sciences,Nanjing,Jiangsu 210014,China)

机构地区：[1]广东省农业科学院植物保护研究所/农业农村部华南果蔬绿色防控重点实验室/广东省植物保护新技术重点实验室,广东广州510640 [2]江苏省农业科学院农业设施装备研究所,江苏南京210014

出　　处：《南方农业学报》2024年第4期1010-1022,共13页Journal of Southern Agriculture

基　　金：国家自然科学基金项目(32001451);广东省基础与应用基础研究基金项目(2021A1515111108)。

摘　　要：【目的】明确种植抗异丙隆转基因水稻和施用除草剂异丙隆对转基因水稻根际微生物群落的影响,为抗异丙隆水稻种植的生态风险评价提供科学依据。【方法】基于盆栽试验,采用高通量测序技术分析2个抗异丙隆转基因水稻株系及其亲本和施用异丙隆转基因在不同水稻生长阶段的根际微生物群落多样性与组成的差异。【结果】高通量测序结果显示,在水稻所有生长阶段,变形菌门(Proteobacteria)、酸杆菌门(Acidobacteria)、芽单胞菌门(Gemmatimonadota)、拟杆菌门(Bacteroidota)和粘球菌门(Myxococcota)是根际微生物中的优势细菌门;罗兹菌门(Rozellomycota)、子囊菌门(Ascomycota)、担子菌门(Basidiomycota)、球囊菌门(Glomeromycota)和被孢霉门(Mortierellomycota)是根际微生物中的优势真菌门。在水稻相同生长阶段,无论是种植抗异丙隆转基因水稻、非转基因水稻还是施用除草剂异丙隆,水稻根际微生物群落的丰富度指数、辛普森多样性指数和香农多样性指数均未受到显著影响(P>0.05,下同);抗异丙隆转基因水稻、施用异丙隆的转基因水稻与非转基因水稻根际微生物中多数优势微生物门类相对丰度无显著差异,个别优势微生物门在水稻特定的生长阶段发生变化。基于相对丰度前15位微生物属的聚类分析显示,在水稻某些生长阶段根际微生物群落的组成在抗异丙隆转基因水稻、施用异丙隆的转基因水稻与非转基因水稻间存在一定差异。根际微生物群落的主成分分析显示,抗异丙隆转基因水稻、施用异丙隆的转基因水稻和非转基因水稻根际微生物群落组成间无显著差异,但不同生长阶段的根际微生物群落组成出现空间分异。根际微生物群落变差分解分析显示,水稻生长阶段对根际土壤中的细菌群落结构变化的影响为45.62%,而品种和施用异丙隆对根际土壤中细菌群落结构变化的影响分别为1.15%和【Objective】The effects of planting isoproturon-resistant transgenic rice and application of herbicide isoproturon on rhizospheric microbial communities of transgenic rice were studied to provide a scientific basis for ecological risk assessment of herbicide-resistant rice cultivation.【Method】Based on pot experiments,the differences in rhizosphere microbial community diversity and composition between two isoproturon-resistant transgenic rice lines and their parents and isoproturon-treated transgenic rice at different rice growth stages were analyzed by high-throughput sequencing technique.【Result】The results of high-throughput sequencing showed that the dominant bacterial phyla in rhizospheric microbial communities at all growth stages of rice were Proteobacteria,Acidobacteriota,Gemmatimonadota,Bacteroidota,and Myxococcota.The dominant fungal phyla in the rhizosphere microorganisms were Rozellomycota,Ascomycota,Basidiomycota,Glomeromycota,and Mortierellomycota.Regardless of the cultivation of isoproturon-resistant transgenic rice,non-transgenic rice or the application of herbicide isoproturon,the richness index,Simpson diversity index,and Shannon diversity index of rice rhizosphere microbial communities were not significantly affected at the same growth stage of rice(P>0.05,the same below).There was no significant difference in the relative abundance of most dominant microbial phyla in the rhizosphere microorganisms of isoproturon-resistant transgenic rice,isoproturon-treated transgenic rice and non-transgenic rice.Individual dominant phyla changed at specific growth stages of rice.The cluster analysis based on the top 15 abundant microbial genera also showed that there were some differences in the composition of rhizospheric microbial communities among isoproturon-resistant transgenic rice,isoproturon-treated transgenic rice,and non-transgenic rice at some growth stages of rice.The principal component analysis of rhizosphere microbial communities showed that there was no significant difference in the

关 键 词：转基因水稻 除草剂 异丙隆 根际微生物群落 高通量测序 

分 类 号：S154.3[农业科学—土壤学] S511[农业科学—农业基础科学]