紫归润唇膏体内外抗炎功效评价  

Evaluation of the anti-inflammatory effect of Zigui lip balm in vitro and in vivo

作　　者：朱聪聪 罗楹 黄梽楠 朱全刚 ZHU Congcong;LUO Ying;HUANG Zhinan;ZHU Quangang(Department of Pharmacy,Shanghai Skin Disease Hospital,Shanghai 200443,China;Shanghai Engineering Research Center for Topical Chinese Medicine,Shanghai 200443,China;Department of Dermatology,Yueyang Hospital of Integrated Traditional Chinese and Western Medicine,Shanghai University of Traditional Chinese Medicine,Shanghai 200437,China;Institute of Dermatology,Shanghai Academy of Traditional Chinese Medicine,Shanghai 201203,China;School of Traditional Chinese Materia Medica,China Pharmaceutical University,Nanjing 211100,China)

机构地区：[1]上海市皮肤病医院药剂科,上海200443 [2]上海中药外用制剂创新工程技术研究中心,上海200443 [3]上海中医药大学附属岳阳中西医结合医院皮肤科,上海200437 [4]上海市中医药研究院皮肤病研究所,上海201203 [5]中国药科大学中药学院,南京211100

出　　处：《上海医药》2024年第11期3-6,22,共5页Shanghai Medical & Pharmaceutical Journal

摘　　要：目的:体内外评价紫归润唇膏的抗炎功效。方法:建立脂多糖诱导的RAW 264.7细胞炎症模型,检测紫归醇提物对一氧化氮生成的影响。建立卡泊三醇诱导的特应性皮炎样小鼠模型,研究紫归润唇膏对小鼠耳皮肤厚度、耳皮肤炎症的严重程度、组织病理及安全性的影响。结果:25~200μg/mL紫归醇提物能够促进RAW 264.7细胞生长且显著降低一氧化氮水平。同时紫归润唇膏能够显著降低特应性皮炎样小鼠耳部厚度,降低湿疹面积及严重度指数(eczema area and severity index,EASI)评分,减少炎症细胞浸润,降低棘层增厚,改善小鼠耳廓皮损炎症情况,且对卡泊三醇诱导的特应性皮炎样小鼠肝脾肾无影响。结论:紫归润唇膏具有显著抗炎功效且具有安全性。Objective:To evaluate the anti-inflammatory effects of Zigui lip balm both in vitro and in vivo.Methods:The model of inflammation was established using lipopolysaccharide-induced RAW 264.7 cells to detect the impact of Zigui alcohol extract on nitric oxide production.The model of atopic dermatitis-like mice induced by capsaicin was established to study the effects of Zigui lip balm on ear skin thickness,severity of ear skin inflammation,histopathology,and safety.Results:Zigui alcohol extract at 25-200 mg/mL could significantly promote the growth of RAW 264.7 cells and reduce nitric oxide levels.Meanwhile,Zigui lip balm could also significantly reduce ear thickness,eczema area and severity index(EASI)scores,inflammatory cell infiltration and decrease epidermal thickening and improve inflammation of the atopic dermatitis-like mice ear skin and in addition,Zigui lip balm had no effect on the liver,spleen,and kidneys of mice with capsaicin-induced atopic dermatitis.Conclusion:Zigui lip balm exhibits significant anti-inflammatory effects and is safe for use.

关 键 词：紫归润唇膏 抗炎 功效评价 

分 类 号：R286[医药卫生—中药学] R275.9[医药卫生—中医学]