紫外线B(UVB)损伤人皮肤基底膜屏障的分子特征  

Molecular characteristics of human skin basement membrane barrier damaged by ultraviolet B(UVB)

作　　者：杨雅麟 刘一洲 王久存[2] 马彦云[2] YANG Yalin;LIU Yizhou;WANG Jiucun;MA Yanyun(School of Life Sciences,Inner Mongolia University,Hohhot 010021,China;School of Life Sciences,Fudan University,Shanghai 200438,China)

机构地区：[1]内蒙古大学生命科学学院,呼和浩特010021 [2]复旦大学生命科学学院,上海200438

出　　处：《上海医药》2024年第11期37-41,56,共6页Shanghai Medical & Pharmaceutical Journal

摘　　要：目的:观察UVB照射引起的基底膜屏障损伤的特征。方法:使用40 mJ/cm^(2)UVB照射HaCaT细胞,通过RNA-seq数据分析研究基底膜屏障相关基因的表达特征。通过透射电镜和免疫荧光染色,在3组离体皮肤组织中观察50 mJ/cm^(2)UVB连续照射3 d后基底膜结构及关键屏障蛋白含量的变化。结果:发现了18个与基底膜相关的基因,其中17个基因表达下调,COL4A4、COL4A5、COL4A6、ITGB1、ITGA3、ITGA4、ITGA6、LAMA5的表达水平下调倍数超过1.5倍(P<0.05),而HPSE的表达上调了2.4倍(P<0.0001)。在离体皮肤组织实验中,UVB照射后观察到基底膜透明层和致密层断裂,半桥粒破坏,完整性和连续性受损,并且整合素蛋白含量下降(P<0.05)。结论:UVB照射会下调基底膜屏障结构相关基因的表达,上调乙酰肝素酶基因表达,造成基底膜结构紊乱以及基底膜关键屏障蛋白降解,使基底膜屏障受损。Objective:To observe the characteristics of basement membrane barrier damage caused by UVB irradiation in cell models and isolated skin tissues.Methods:HaCaT cells were irradiated with 40 mJ/cm^(2) UVB,and the expression characteristics of basement membrane barrier-related genes were analyzed by RNA-seq data.In addition,transmission electron microscopy and immunofluorescence staining were used to observe the changes of basement membrane structure and key barrier protein content in three groups of isolated skin tissues after continuous irradiation with 50 mJ/cm^(2) UVB for 3 days.Results:Eighteen genes related to basement membrane were found,of which the expression of 17 genes were down-regulated.The expression levels of COL4A4,COL4A5,COL4A6,ITGB1,ITGA3,ITGA4,ITGA6 and LAMA5 was down-regulated by more than 1.5 times(P<0.05),while the expression level of HPSE was up-regulated by 2.4 times(P<0.0001).In the in vitro skin tissue experiment,the transparent layer and dense layer of the basement membrane were observed to be broken after UVB irradiation,the hemidesmosomes were destroyed and their integrity and continuity were damaged,and the integrin protein content was decreased(P<0.05).Conclusion:UVB irradiation can down-regulate the expression of genes related to the structure of the basement membrane barrier,up-regulate the expression of heparanase gene,cause the disorder of the basement membrane structure and the degradation of the key barrier protein of the basement membrane,and damage the basement membrane.

关 键 词：UVB 离体皮肤组织培养 皮肤基底膜损伤 

分 类 号：R339.57[医药卫生—人体生理学] R751[医药卫生—基础医学]