一种特异性识别Cys的近红外荧光探针  

A Fluorescent Probe for Specific Recognition of Cys Using Near-Infrared Technology

作　　者：孟祥聪 MENG Xiangcong(Jiangxi Science and Technology Normal University,Nanchang Jiangxi 330038,China)

机构地区：[1]江西科技师范大学,江西南昌330038

出　　处：《江西化工》2024年第3期20-25,共6页Jiangxi Chemical Industry

基　　金：国家自然科学基金(22261020、21861017、41867053);江西省教育厅科学基金项目(GJJ2201331);江西科技师范大学博士启动基金(2022BSQD35)。

摘　　要：半胱氨酸作为生物体内一种非必需氨基酸,存在于各种蛋白质和肽中,当其含量出现异常波动时便会导致各种疾病发生。本研究将修饰的苯并吡喃盐作为近红外发色团,以3-氯丙酰酯为识别基团连接构建了一种特异性识别Cys的线粒体靶向荧光探针BP-L。BP-L中的3-氯丙酰酯与Cys经过取代-环化-离去反应,使化学环境发生变化从而导致荧光产生。BP-L实现了“裸眼”检测,具有较低细胞毒性。本文将其应用于细胞共定位成像,为相关生物活体检测提供了可能性。Cysteine is a non-essential amino acid found in various proteins and peptides in living organisms.Abnormal fluctuations in its content can lead to the development of various diseases.To specifically recognize Cys,a mitochondria-targeted fluorescent probe,BP-L,was constructed by linking a modified benzopyran salt as a near-infrared chromophore and 3-chloropropionyl ester as a recognition group.The substitution-cyclization-dissociation reaction between 3-chloropropionyl ester in BP-L and Cys causes a change in the chemical environment,resulting in fluorescence generation.BP-L enables‘naked-eye’detection with low cytotoxicity and has been utilized for co-localized cellular imaging,offering the potential for in vivo detection of relevant organisms.

关 键 词：苯并毗喃盐 线粒体定位 半胱氨酸 近红外荧光探针 细胞成像 

分 类 号：O657.33[理学—分析化学]