新型导电填料加速形成厌氧氨氧化生物膜及脱氮效能研究  

Accelerate Formation of Anammox Biofilm Using New Conductive Fillers and Its Nitrogen Removal Efficiency

作　　者：杨海龙 马少杰 王卓 丁建婷 王朝霞 邱瑞芳[3] 吉莉[2] 谢飞 YANG Hai-long;MA Shao-jie;WANG Zhuo;DING Jian-ting;WANG Zhao-xia;QIU Rui-fang;JI Li;XIE Fei(Shanxi Provincial Institute of Ecological Environment Planning and Technology,Taiyuan 030000,China;School of Environment and Resources,Taiyuan University of Science and Technology,Taiyuan 030024,China;School of Environment and Resources,Shanxi University,Taiyuan 030006,China)

机构地区：[1]山西省生态环境规划和技术研究院,太原030000 [2]太原科技大学环境与资源学院,太原030024 [3]山西大学环境与资源学院,太原030006

出　　处：《科学技术与工程》2024年第18期7921-7927,共7页Science Technology and Engineering

基　　金：国家自然科学基金(52270134);山西省应用基础研究(青年科技)项目(202203021212299);太原科技大学科研启动基金(20222047)。

摘　　要：厌氧氨氧化菌(anaerobic ammonia oxidizing bacteria,AnAOB)在实际脱氮工艺中持留能力差,是制约该技术规模化应用的主要技术瓶颈之一。相较于传统生物填料,高温热解轮胎制备成具有导电特性的新型生物填料,不仅可以回收利用固定废弃物,而且能够实现加速AnAOB形成生物膜和提高脱氮效率的双重作用。实验结果表明,800℃最适合用于热解轮胎填料,同时具备更高的电导率和更有利于固定微生物生长特性。相较于单独Anammox组,Anammox+导电填料的NH+4-N、NO-2-N和TN平均去除率比单独Anammox组分别提升了9.12%、13.20%和15.69%,且明显高于聚丙烯(polypropylene,PP)填料的强化效果。Q-PCR分析结果表明,添加导电填料能够促使脱氮菌种丰度增加,其中AnAOB、亚硝酸盐还原菌和硝酸盐还原菌分别增长了50.21%、69.70%和145.59%。该新型导电填料技术的研发,可为Anammox技术推广提供新的思路和数据基础。Anaerobic ammonia oxidizing bacteria(AnAOB)have poor retention capacity in actual denitrification processes,which is one of the main technical bottlenecks restricting the large-scale application of this technology.Compared with traditional biological fillers,high-temperature pyrolysis tires can be used to prepare a new type of biological filler with conductive properties.This not only recycles fixed waste,but also achieves a dual effect of accelerating the formation of biofilm in AnAOB and improving denitrification efficiency.The experimental results indicate that 800℃is the most suitable for pyrolysis tire fillers,while having higher electrical conductivity and more favorable characteristics for fixed microbial growth.Compared to the Anammox group alone,the average removal rates of NH+4-N,NO-2-N,and TN with Anammox+conductive filler increased by 9.12%,13.20%,and 15.69%,respectively,compared to the Anammox group alone,and were significantly higher than the strengthening effect of polypropylene(PP)filler.The Q-PCR analysis results showed that adding conductive fillers can promote an increase in the abundance of denitrifying bacteria,with AnAOB,nitrite reducing bacteria,and nitrate reducing bacteria increasing by 50.21%,69.70%,and 145.59%,respectively.The development of this new conductive filler technology can provide new ideas and data basis for the promotion of Anammox technology.

关 键 词：厌氧氨氧化 导电填料 废弃轮胎 厌氧生物膜 

分 类 号：X703[环境科学与工程—环境工程]