青海省春油菜主推品种的指纹图谱构建和纯度鉴定  

Construction of Fingerprint and Purity Identification of Main Cultivars of Spring Brassica napus in Qinghai Province

作　　者：王国邦 谢立人 杜德志[1] 李开祥[1] WANG Guobang;XIE Liren;DU Dezhi;LI Kaixiang(Academy of Agricultural and Forestry Sciences,Qinghai University/Laboratory for Research and Utilization of Qinghai Tibet Plateau Germplasm Resources/Key Laboratory of Spring Rapeseed Genetic Improvement of Qinghai Province/Qinghai Research Branch of the National Rapeseed Genetic Improvement Center,Xining 810016,China)

机构地区：[1]青海大学农林科学院/青藏高原种质资源研究与利用实验室/青海省春油菜遗传改良重点实验室/国家油菜改良中心青海分中心,西宁810016

出　　处：《种子》2024年第6期21-27,共7页Seed

基　　金：青海省帅才科学家负责制项目(2022-NK-170)。

摘　　要：在190对甘蓝型油菜SSR引物中筛选出具有多态性、条带清晰、重复性好的16对SSR标记,使用16对核心引物对青杂系列春油菜品种的15份亲本材料进行指纹图谱构建。结果表明,16个标记共扩增出44个位点,每对引物平均扩增的位点为2.5个,使用UPGMA法将15份材料在遗传距离为0.65处分为三类。同时利用其中的两对SSR标记(A04-10、A06-10)、与波里马细胞质雄性不育恢复基因(Rfp)紧密连锁的标记YSSR3对青杂4号、青杂7号、青杂有限1号、青杂5号、青杂9号、青杂15号、青杂12号、青杂16号、青杂18号的杂交种进行纯度鉴定,表明SSR标记用于杂交种的纯度鉴定更有优势。A total of 16 SSR markers with polymorphism,clear bands and good repeatability were selected from 190 pairs of Brassica napus SSR primers.16 pairs of core primers were used to construct fingerprints of 15 parent materialSof spring Brassica napus varieties.The results showed that a total of 44 loci were amplified by 16 markers,with an average amplification of 2.5 loci per primer pair.The 15 materialSwere classified into three classes by UPGMA at a genetic distance of 0.65.Two pairs of SSR markers (A04-10,A06-10)were used,and the markers were closely linked to the Polymaa cytoplasmic male sterility restoration gene(Rfp)YSSR3 wasused to identify the purity of the hybrids of Qingza No.4,Qingza No.7,Qingza-limited No.1,Qingza No.5,Qingza No.9,Qingza No.15,Qingza No.12,Qingza No.16 and Qingza No.18,which showed that SSR markers were more advantageouSfor the purity identification of hybrids.

关 键 词：甘蓝型油菜 SSR标记 指纹图谱 纯度鉴定 

分 类 号：S565.4[农业科学—作物学]