四倍体硬粒小麦及其他小麦族AP2/ERF基因家族比较分析及表达鉴定  

Comparative Analysis and Expression Identification of the AP2/ERF Gene Family in Tetraploid Triticum turgidum subsp.durum and other Wheat Families

作　　者：张新 李敏敏 孙允超 赵杨 冀传允 许传怀 闫树平 张兰迎 张继勇 ZHANG Xin;LI Minmin;SUN Yunchao;ZHAO Yang;JI Chuanyun;XU Chuanhuai;YAN Shuping;ZHANG Lanying;ZHANG Jiyong(Liaocheng Academy of Agricultural Sciences,Liaocheng Shandong 252000,China;Shandong Dixin Agricultural Technology Co.,Ltd.,Liaocheng Shandong 252000,China)

机构地区：[1]聊城市农业科学院,山东聊城252000 [2]山东帝芯农业科技有限公司,山东聊城252000

出　　处：《种子》2024年第6期55-66,85,共13页Seed

基　　金：国家小麦产业技术体系(CARS-3);山东省现代农业产业技术体系小麦创新团队(SDAIT-01);聊城市重点研发计划:小麦种质创新利用与新品种选育(2023YD91)。

摘　　要：为探究硬粒小麦及其他小麦族中AP2/ERF家族基因响应非生物胁迫的机理,本研究采用生物信息学的方法在全基因组范围内对硬粒小麦、野生二粒小麦及粗山羊草的已有公开数据进行分析,分别鉴定了215、232、139个AP2/ERF基因家族成员,AP2/ERF家族基因在不同物种中的基因组位置、基因结构、启动子顺式作用元件相似及差异部分。利用硬粒小麦在不同发育时期及非生物胁迫下的转录组数据,对这些AP2/ERF基因进行表达模式分析,发现有6个AP2/ERF基因在硬粒小麦胚乳发育过程中起着一定程度的调节作用;有23个AP2/ERF基因在硬粒小麦花后热胁迫反应中上调表达,其中13个基因可以同时响应硬粒小麦花前缺水及花后热胁迫反应。In order to explore the mechanism of AP2/ERF family genes in durum wheat and other wheat families in response to abiotic stress,bioinformatics methods were used to analyze the published data of Triticum turgidum subsp.durum,Triticum turgidum var.dicoccoideSand AegilopStauschii in the whole genome.215,232 and 139 memberSof AP2/ERF gene family in different species were identified.The similarity and difference of AP2/ERF gene family in genome location,gene structure and promoter cis-acting elementSwere also identified.The expression patternSof these AP2/ERF genes were analyzed using the transcriptome data of Triticum turgidum subsp.durum at different developmental stageSand under abiotic stress.6 AP2/ERF genes were found to play a certain role in regulating the endosperm development of Triticum turgidum subsp.durum.There were 23 AP2/ERF genes up-regulated in postflowering heat stress response of Triticum turgidum subsp.durum,13 of which could respond to both pre-flowering water shortage and postflowering heat stress response of Triticum turgidum subsp.durum.

关 键 词：硬粒小麦 AP2/ERF基因家族 非生物胁迫 表达模式 

分 类 号：S512.1[农业科学—作物学]