不同培养基对固沙先锋植物羽毛针禾种子萌发的影响  

The Effect of Different Culture Media on the Germination of the Sand Fixing Pioneer Plant Stipagrostis pennata Seeds

作　　者：郭楠 陈港 马红霞 孙雨 胡鸣 王斐[1,2,3] 李榕 李鸿彬[1,2,3] GUO Nan;CHEN Gang;MA Hongxia;SUN Yu;HU Ming;WANG Fei;LI Rong;LI Hongbin(School of Life Sciences,Shihezi University,Shihezi Xinjiang 832003,China;Key Laboratory of Oasis Urban and Mountain Basin System Ecology Corps,Shihezi Xinjiang 832003,China;Key Laboratory of Plant Medicine Resources Utilization,Ministry of Education,Shihezi Xinjiang 832003,China)

机构地区：[1]石河子大学生命科学学院,新疆石河子832003 [2]绿洲城镇与山盆系统生态兵团重点实验室,新疆石河子832003 [3]新疆植物药资源利用教育部重点实验室,新疆石河子832003

出　　处：《种子》2024年第6期146-152,共7页Seed

基　　金：国家自然科学基金项目(32060082);兵团科技计划项目(2020BC002);石河子大学科技计划项目(KX008505);促进与加拿大、澳大利亚、新西兰及拉美地区科研合作与高层次人才培养项目。

摘　　要：为探索固沙先锋植物羽毛针禾种子萌发的最适培养基条件,以羽毛针禾种子为材料,利用MS、N6、1/2 MS、琼脂和White等5种培养基进行羽毛针禾种子萌发实验。在筛选出高效(琼脂培养基)和低效(White)培养基后,对种子进行可溶性糖含量、可溶性蛋白含量、α-淀粉酶活性以及赤霉素、脱落酸、乙烯利、茉莉酸和生长素等萌发相关指标的检测。结果表明,琼脂培养基中种子萌发率最高,种子萌发前可溶性蛋白含量和α-淀粉酶活性持续升高,可溶性糖含量基本保持稳定,相关植物激素赤霉素、脱落酸、乙烯利、茉莉酸和生长素含量处于持续上升的状态。White培养基中萌发率最低,种子萌发前α-淀粉酶活性持续升高,可溶性蛋白含量显著下降,赤霉素浓度显著低于琼脂糖培养基,脱落酸的含量高于琼脂糖培养基。研究结果表明,琼脂培养基为5种羽毛针禾种子萌发的最高效培养基。In order to explore the optimum medium conditions for seed germination of Stipagrostis pennata,a pioneer plant of sand fixation,MS,N6,1/2 MS,AGAR and White media were used for seed germination experiments.After high efficiency(AGAR medium)and low efficiency(White)medium were selected,the seeds were tested for soluble sugar,soluble protein,α-amylase,germinal,ababolic acid,ethethylene,jasmonic acid and growth hormone.The results showed that the seed germination rate was the highest in AGAR medium.Before germination,soluble protein content andα-amylase activity continued to increase,soluble sugar content remained stable,and the contents of related plant hormones gibberellin,ababolic acid,ethethylene,jasmonic acid and auxin continued to increase.Before germination,α-amylase activity continued to increase,soluble protein content decreased significantly,gibberellin concentration was significantly lower than agarose medium,and abscisic acid content was higher than agarose medium.The results showed that AGAR medium was the most efficient medium for seed germination of 5 species of Stipagrostis pennata.

关 键 词：羽毛针禾 禾本科种子萌发 培养基 代谢 酶活性 内源激素 

分 类 号：Q948[生物学—植物学]