碱性副品红染色法检测木质部导管栓塞技术改进  

Detection by Staining of Embolic Vessels of Robinia pseudoacacia Branch

作　　者：张玲玲[1] ZHANG Linging(College of Forestry,Northwest Agriculture and Forestry University,Shannxi,Xi,an 712100)

机构地区：[1]西北农林科技大学林学院,陕西西安712100

出　　处：《浙江林业科技》2024年第3期74-80,共7页Journal of Zhejiang Forestry Science and Technology

摘　　要：染色法是测量导管长度、直径、导管分布以及栓塞脆弱性的重要方法。在测量导管栓塞时,染色压力和时间条件将极大地影响实验结果的准确性。为准确利用染色法测定刺槐Robinia pseudoacacia导管栓塞状况,本研究通过使用充气组(人为栓塞100%)以及冲洗组(完全去除栓塞)对碱性副品红染色法进行了压力、时间条件的改良。结果表明,当压力在0.04kpa,染色时间60min时,实验结果最为准确可靠。在此压力下染色过程不会冲开栓塞导管,配合60 min的染色时间可对未栓塞导管进行充分染色。另外,本研究通过诱导组,利用离心机法对枝条诱导不同程度的栓塞与染色法进行了对比验证,结果表明在该染色条件下,可以很好地反映茎段的栓塞状况,保证了实验结果的准确性。在后续试验中,使用碱性副品红染色法研究木质部栓塞时可以沿用此压力及时间条件。Collection of 2-year branches of 5-year Robinia pseudoacacia was carried out in Yangling,Shaanxi province.Experiment was conducted on embolism detection by staining on artificial embolic vessel group(100%),de-embolic vessel group(removing embolism completely)with different pressure and duration.The result demonstrated that it had the best effect of detection with the pressure of 0.04 kpa,duration of 60 minutes.With that combination,embolic vessel group would not be dyed while de-embolic vessel group could be dyed completely.Comparison on detection result with different negative pressure showed that it could be detected accurately.

关 键 词：木质部 刺槐 栓塞 染色法 

分 类 号：S758.8[农业科学—森林经理学]