基于中空纤维的灌流培养细胞截留技术的研究  

Study on cell retention technologies of perfusion culture based on hollow fiber

作　　者：徐聪 贾辉[1] 秦卿雯 韩永良 白静娜 李雯 安子韩 王捷[1] XU Cong;JIA Hui;QIN Qingwen;HAN Yongliang;BAI Jingna;LI Wen;AN Zihan;WANG Jie(School of Environmental Science and Engineering,Tiangong University,Tianjin 300387,China;School of Environmental Engineering,Henan University of Technology,Zhengzhou 450001,China;Tianjin CNCLEAR Environmental Protection Technology Co.,Ltd,Tianjin 300401,China;The Institute of Seawater Desalination and Multipurpose Utilization,MNR(Tianjin),Tianjin 300192,China)

机构地区：[1]天津工业大学环境科学与工程学院,天津300387 [2]河南工业大学环境工程学院,郑州450001 [3]天津汉晴环保科技有限公司,天津300401 [4]自然资源部天津海水淡化与综合利用研究所,天津300192

出　　处：《膜科学与技术》2024年第3期185-192,共8页Membrane Science and Technology

基　　金：天津市科技创新计划(22ZXSYSY00010)。

摘　　要：近年来由于生物制药行业对于蛋白产品需求的日益增加,连续型细胞培养工艺的灌流培养模式越来越受到重视.为了维持生物反应器中较高的细胞数量和生产力,收获纯度较高的蛋白产品,需要一种良好的分离装置来保留生物反应器中的细胞.本文在比较不同类型的细胞截留技术的基础上,总结了中空纤维过滤技术操作条件温和、通量大、过滤精度高且应用广泛的优势.为了缓解中空纤维过滤过程中存在的膜污染问题,延长膜组件寿命,本文还分析了灌流培养常用的中空纤维膜材质、孔径大小、组件内径、长度及填充密度等设计因素,讨论了不同的切向流过滤操作参数对膜组件的影响,以期为灌流培养工艺领域中细胞分离用中空纤维膜组件的设计与优化提供理论依据.Recently,due to the increasing demand for protein products in the bio-pharmaceutical industry,the perfusion culture mode of continuous cell culture technology has been increasingly valued.To maintain a high number of cells and productivity in bioreactors and harvest high-purity protein products,a good separation device is needed to retain the cells in the bioreactor.Based on comparing different types of cell retention technologies,this article summarizes the advantages of hollow fiber filtration technologies,such as mild operating conditions,high flux,high filtration accuracy,and wide application.In order to alleviate the membrane fouling problem in the process of hollow fiber filtration and extend the lifespan of membrane modules,this article also analyzes the design factors such as hollow fiber material,pore size,module inner diameter,length,and packing density commonly used in perfusion culture,and discusses the influence of different tangential flow filtration operation parameters on membrane modules.To provide a theoretical basis for the design and optimization of hollow fiber membrane modules for cell separation in the field of perfusion culture technology.

关 键 词：灌流培养 中空纤维膜组件 细胞截留 切向流过滤 组件优化 

分 类 号：TQ028[化学工程] Q819[生物学—生物工程]