鉴定一起人细小病毒疫情中B19病毒的基因特征  

Identification of genetic characteristics of B19 virus in a human parvovirus outbreak

作　　者：张婷[1] 曾其莉[1] 蔡昆[1] ZHANG Ting;ZENG Qili;CAI Kun(Hubei Provincial Center for Disease Control and Prevention,Wuhan,Hubei 430079,China)

机构地区：[1]湖北省疾病预防控制中心,湖北武汉430079

出　　处：《公共卫生与预防医学》2024年第4期45-48,共4页Journal of Public Health and Preventive Medicine

基　　金：湖北省自然科学基金面上项目(2020CFB516);国家自然科学基金区域创新发展联合基金重点项目(U21A20423);病毒学国家重点实验室开放研究基金(2022KF001);湖北省公共卫生青年拔尖人才项目;中华预防医学会消毒科研项目(XD2022-Z-03)。

摘　　要：目的 分析2019年湖北省一起人细小病毒疫情中B19细小病毒的基因特征,为预防控制细小病毒暴发疫情提供科学依据。方法 收集2019年湖北省一起B19暴发疫情中的血清样本17份,咽拭子样品20份。采用荧光定量RT-PCR方法筛选出核酸阳性标本,用巢式PCR扩增B19阳性标本的NS1-VP1u区(1 157bp)的特定序列,确定B19基因型别。结果 17例病人样本的B19病毒基因型别为1a亚型,核苷酸序列同源性为99.9%~100%。B19病毒1a基因亚型与中国北京株KR819772、中国北京株KR819773、中国杭州株KT310174位于同一小分支中,距离最近。结论 2019年湖北省一起人细小病毒暴发中的B19病毒属于1a亚型。Objective To analyze the genetic characteristics of B19 parvovirus in a human parvovirus outbreak in Hubei Province in 2019,and to provide scientific basis for the prevention and control of parvovirus outbreaks.Methods Among of the 17 serum specimens and 20 throat swab specimens from a parvovirus B19 outbreak were collected in Hubei Province in 2019.The nucleic acid-positive specimens were identified by fluorescence quantitative RT-PCR.The specific sequences of NS1-VP1u(1 157 bp) of B19 positive specimens were amplified by nested PCR to determine B19 genotype.Results The genotype of B19 virus in 17 patients were subgenotype 1a,and the nucleotide homology of subgenotype 1a was 99.90%-100%.The B19 subgenotype 1a was located in the same small branch as the Chinese Beijing strain KR819772,the Chinese Beijing strain KR819773 and the Chinese Hangzhou strain KT310174,and the distance was the closest.Conclusion The B19 virus in a human parvovirus outbreak in Hubei Province in 2019 belongs to subgenotype 1a.

关 键 词：人细小病毒 基因型别 核苷酸 同源性 

分 类 号：R181[医药卫生—流行病学]