不同水力停留时间对新疆油田压裂返排液COD去除及微生物菌群特征研究  

The research of COD removal and microbiological community characteristics of Xinjiang oilfield fracturing flowback fluid with different hydraulic retention times

作　　者：谢鲲鹏[1] 于思慧 罗红静 张永胜 马畅蔚 胡浩宇 宫正[1] XIE Kunpeng;YU Sihui;LUO Hongjing;ZHANG Yongsheng;MA Changwei;HU Haoyu;GONG Zheng(School of Life Sciences,Liaoning Normal University,Dalian 116081,China)

机构地区：[1]辽宁师范大学生命科学学院,辽宁大连116081

出　　处：《辽宁师范大学学报（自然科学版）》2024年第2期203-212,共10页Journal of Liaoning Normal University:Natural Science Edition

基　　金：辽宁师范大学横向课题(H2022011)。

摘　　要：针对新疆油田压裂返排液较难处理的现状,采用生物膜法作为处理工艺的主要单元,探究不同水力停留时间(HRT)对化学需氧量(COD)去除的影响,通过高通量测序和功能预测确定不同水力停留时间下的微生物群落特征.结果表明:水力停留时间48 h是生物膜法处理新疆油田压裂返排液的适宜水力停留时间,COD去除效率最高,进水COD平均去除率46.77%,平均去除速率83.33 mg·(L·d)^(-1).相对于水力停留时间36、72 h,水力停留时间48 h的微生物多样性更高,群落组成更丰富,且形成以Proteobacteria(变形菌门)、Firmicutes(厚壁菌门)、Campylobacterota(弯曲菌门)、Bacteroidota(拟杆菌门)和Desulfobacterota(脱硫菌门)为主要优势菌的微生物群落.微生物菌群具有难降解物质代谢的功能基因,可促进新疆油田压裂返排液的生物降解.In allusion to the current situation of Xinjiang oilfield fracturing flowback fluid,which is difficult to treat,the biofilm method was adopted as the main unit of the treatment process to investigate the influence of different hydraulic retention times(HRT)on chemical oxygen demand(COD)removal.The microbiological community characteristics were determined by high-throughput sequencing and functional prediction under different hydraulic retention times.The results showed that the suitable HRT was 48 h when biofilm method was used to treat the fracturing return fluid in the Xinjiang oilfield.The COD removal efficiency was the highest,the average removal rate of the influent COD was 46.77%and the average removal velocity was 83.33 mg·(L·d)^(-1).Compared with the HRT of 36 h and 72 h,the microbial diversity of the HRT of 48 h was higher and the community composition was richer.The microbiological community was formed with Proteobacteria,Firmicutes,Campylobacterota,Bacteroidota,and Desulfobacterota as the main dominant bacteria under the HRT of 48 h.The microflora had functional genes for the metabolism of difficult-to-biodegrade substances and promote the biodegradation of fracturing flowback fluid in Xinjiang oilfield.

关 键 词：水力停留时间 压裂返排液 COD去除 微生物群落 

分 类 号：Q939.97[生物学—微生物学]