高寒岩溶土壤微生物群落组成及其多样性  

Composition and diversity of microbial communities in high-altitude karst soil

作　　者：夏热克亚木·伊提尼牙孜 董发勤[2,3] 李琼芳[1] 安德军[4] 代群威[3,5] 张强[6] 饶瀚云 任亚珍 刘凤起 刘明学[1] XIAREKEYAMU·Yitiniyazi;DONG Faqin;LI Qiongfang;AN Dejun;DAI Qunwei;ZHANG Qiang;RAO Hanyun;REN Yazhen;LIU Fengqi;LIU Mingxue(School of Life Science and Engineering,Southwest University of Science and Technology,Mianyang,Sichuan 621010,China;Key Laboratory of Solid Waste Treatment and Resource Recycle,Ministry of Education,Southwest University of Science and Technology,Mianyang,Sichuan 621010,China;School of Environment and Resources,Southwest University of Science and Technology,Mianyang,Sichuan 621010,China;Huanglong National Scenic Area Administration,Songpan,Sichuan 624000,China;Fundamental Science on Nuclear Wastes and Environmental Safety Laboratory,Southwest University of Science and Technology,Mianyang,Sichuan 621010,China;Institute of Karst Geology,CAGS/Key Laboratory of Karst Dynamics,MNR&GZAR/International Research Center on Karst under the Auspices of UNESCO,Guilin,Guangxi 541004,China)

机构地区：[1]西南科技大学生命科学与工程学院,四川绵阳621010 [2]西南科技大学固体废物处理与资源化教育部重点实验室,四川绵阳621010 [3]西南科技大学环境与资源学院,四川绵阳621010 [4]黄龙国家级风景名胜区管理局,四川松潘624000 [5]西南科技大学核废物与环境安全国防重点学科实验室,四川绵阳621010 [6]中国地质科学院岩溶地质研究所/自然资源部、广西岩溶动力学重点实验室/联合国教科文组织国际岩溶研究中心,广西桂林541004

出　　处：《中国岩溶》2024年第2期364-378,共15页Carsologica Sinica

基　　金：国家自然科学基金-区域创新发展联合基金(U21A2016);国家自然科学基金项目(41572035,41877288,41831285)。

摘　　要：高寒岩溶是四川黄龙风景区独特的地质特征。为分析高寒岩溶区土壤微生物群落组成特征与土壤理化性质间的相关性,以黄龙风景区土壤为研究对象,对土壤细菌的16SrRNA基因序列和真菌ITS序列进行高通量测序。结果表明:不同岩溶区的土壤细菌多样性和丰富度具有显著差异,但土壤真菌差异不显著,且土壤细菌群落占主导地位;细菌群落以变形菌门(Proteobacteria)、酸杆菌门(Acidobacteria)为主;真菌群落以子囊菌门(Ascomycota)、担子菌门(Basidiomycota)为主,真菌在门和属水平的差异较大。冗余分析发现总磷和温度是黄龙风景区土壤微生物群落结构变化的重要环境因子,pH是第二重要的环境因子。As a World Natural Heritage Site,Huanglong Scenic Area,is located in the eastern part of the Qinghai–Tibet Plateau in Songpan county,Aba Tibetan and Qiang Autonomous Prefecture,Sichuan Province.Huanglong valley is 3.50 km long,with an altitude of 3,145–3,578 m.In the Huanglong Scenic Area,there is a sixmonth freezing period,with a minimum temperature of 3℃.The main vegetation types are coniferous and broadleaved mixed forests and coniferous forests,belonging to a typical plateau temperate to subfrigid monsoon climate.This scenic area is renowned at home and abroad for its rare and colorful karst landscape.At present,there is still a research gap in the structure and function of soil microorganisms of high-altitude karst habitats.Therefore,in order to explore the characteristics of soil microbial communities in Huanglong Scenic Area−a high-altitude karst area,we compared the differences in soil microbial community structure and diversity between high-altitude karst and non-highaltitude karst areas,with typical primary karst in Guilin−a non-high-altitude karst area.By analyzing the structural characteristics of soil microbial communities and their correlation with environmental impact factors,we laid a theoretical foundation for the relationship between soil microbial communities and ecological environment in highaltitude karst areas.In this study,we collected and analyzed soil samples from Huanglong valley,the main scenic area of the Huanglong Scenic Area.A total of 27(three replicates)samples were collected and compared with the samples from the typical primary karst area in Guilin.We collected data on environmental factor,such as Total Organic Carbon(TOC),Soil Organic Matter(SOM),Total Nitrogen(TN),Total Product(TP),Temperature(T),Soil Water Content(SWC),and pH.Meanwhile,we employed high-throughput sequencing techniques such as 16SrRNA and ITS gene sequencing to perform bioinformatics analysis on sequencing data,including species diversity andβdiversity.Then,we identified the main driving factors affe

关 键 词：高寒岩溶 黄龙风景区 岩溶土壤 高通量测序 微生物群落结构 环境因子 

分 类 号：S154.3[农业科学—土壤学]