光谱成像技术及其在植物中的应用研究进展  

Spectral imaging technology and its research progress in plants

作　　者：李若凡 葛颜锐 陈盈盈 吴丁洁 崔亚宁[1,2,3,4] 李瑞丽 LI Ruofan;GE Yanrui;CHEN Yingying;WU Dingjie;CUI Yaning;LI Ruii(State Key Laboratory of Tree Genetics and Breeding,College of Biological Sciences and Technology,Beijing Forestry University,Beijing 100083;National Engineering Laboratory for Tree Breeding,College of Biological Sciences and Technology,Beijing Forestry University,Beijing 100083;Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants,Ministry of Education,College of Biological Sciences and Technology,Beijing Forestry University,Beijing 100083;The Tree and Ornamental Plant Breeding and Biotechnology Laboratory of National Forestry and Grassland Administration,College of Biological Sciences andTechnology,Beijing Forestry University,Beijing 100083,China)

机构地区：[1]北京林业大学生物科学与技术学院林木遗传育种全国重点实验室 [2]北京林业大学生物科学与技术学院林木育种与生态修复国家工程研究中心 [3]北京林业大学生物科学与技术学院林木、花卉遗传育种教育部重点实验室 [4]北京林业大学生物科学与技术学院树木花卉育种生物工程国家林业和草原局重点实验室,北京100083

出　　处：《电子显微学报》2024年第3期390-404,共15页Journal of Chinese Electron Microscopy Society

基　　金：国家自然科学基金资助项目(Nos.32270368,31970182);科技创新2030-重大项目(2023ZD04069);北京林业大学优秀研究生导师团队项目(No.YJSY-DSTD2022005);北京林业大学“5·5工程”科研创新团队项目(No.BLRC2023C06);大学生创新创业训练计划(Nos.S202310022067,X202310022120).

摘　　要：光谱成像技术能够同时获取样品的空间与化学信息,是研究复杂生物样品的有力工具。近年来,由于具备非入侵、无损测量等优势,光谱成像技术在食品与农产品检测、生物医学与环境监测等方面引起了越来越多的关注。同时,随着成像技术的发展,光谱成像技术在成像速度、波段数量、光谱分辨率、空间分辨率等方面的性能都得到了极大的提升,空间分辨率甚至可以打破衍射极限达到超分辨级别。本综述总结了拉曼光谱、红外光谱和近红外光谱等几种主要光谱成像技术的基本原理与性能,重点介绍了这类技术在植物细胞壁与木材研究、细胞结构与成分可视化、生物与非生物胁迫检测以及种子的无损测量等领域中的应用进展,并对这一类技术的发展潜力进行了探讨与总结,为今后光谱成像技术在植物研究领域的进一步发展与应用提供理论参考。Spectral imaging technology can obtain both spatial and chemical information of samples.It is a powerful tool to study complex biological samples.Due to non-invasive and non-destructive advantages,spectral imaging technology has attracted great attention in the fields of food and agricultural product detection,biomedical and environmental detection.With the development of imaging technology,the performance of spectral imaging technology in imaging speed,number of bands,spectral resolution,spatial resolution,and other aspects has been greatly improved.Spatial resolution can even break the diffraction limit to realize the super-resolution level.This review summarized the basic principles and performance of several major spectral imaging technologies,including Raman spectroscopy,infrared spectroscopy,and near-infrared spectroscopy.Special attention was paid on the latest application of these technologies in the fields of plant cell wall and wood research,cellular structure and composition visualization,biological and abiotic stress detection,and non-destructive measurement of seeds.The development potential of these technologies was summarized,which can provide theoretical references for the further development and application of spectral imaging technologies in the field of plant research in the future.

关 键 词：成像 拉曼光谱 红外光谱 近红外光谱 细胞壁 细胞结构 

分 类 号：Q-336[生物学] Q2-33Q942