母乳喂养的高胆红素血症足月新生儿肠道菌群结构变化  

Changes of gut microbiota structure in breast-fed full-term neonates with hyperbilirubinemia

作　　者：李国彬 何晨曦 范琪 谢秀春 王苗 郭小霞 于瑛 郭万鑫 陈艳妮[4] 冯爱民 LI Guobin;HE Chenxi;FAN Qi;XIE Xiuchun;WANG Miao;GUO Xiaoxia;YU Ying;GUO Wanxin;CEHN Yanni;FENG Aimin(The First Clinical Medical College,Shaanxi University of Traditional Chinese Medicine,Xianyang 712046,China;不详)

机构地区：[1]陕西中医药大学第一临床医学院,陕西咸阳712046 [2]西安交通大学附属儿童医院东院区儿科 [3]陕西中医药大学附属医院儿科 [4]西安交通大学附属儿童医院发育行为科

出　　处：《山东医药》2024年第18期11-15,共5页Shandong Medical Journal

基　　金：陕西中医药大学中西医结合防治脑发育障碍性疾病创新团队项目(2019-YL07)。

摘　　要：目的观察母乳喂养的高胆红素血症足月新生儿的肠道菌群结构变化。方法40例母乳喂养的足月新生儿,根据是否发生高胆红素血症分为高胆红素血症12例(高胆组)、非高胆红素血症28例(对照组),分别采集两组新生儿出生24 h内首次胎便及出生第3~5天时粪便标本,采用16S rDNA基因测序技术检测肠道菌群,采用β多样性分析两组肠道菌群物种多样性,采用关键物种差异分析两组肠道菌群的组成差异。结果出生24 h内高胆红素血症组、对照组新生儿肠道菌群β多样性结果分别为0.51(0.44,0.61)、0.53(0.42,0.65),出生第3~5天时高胆红素血症组、对照组新生儿肠道菌群β多样性结果分别为0.41(0.28,0.49)、0.41(0.32,0.52),与出生24 h内相比,出生第3~5天时对照组新生儿肠道菌群β多样性高(P<0.05)。在属水平上,出生24 h内高胆红素血症组新生儿肠道菌群以埃希氏杆菌属、水杆菌属及污泥单胞菌属等为主,对照组以水杆菌属、污泥单胞菌属及狭义梭菌属等为主;出生第3~5天时高胆红素血症组新生儿肠道菌群以埃希氏杆菌属、狭义梭菌属及链球菌属等为主,对照组以狭义梭菌属、埃希氏杆菌属及拟杆菌属等为主。在门水平上,出生24 h内高胆红素血症组及对照组新生儿肠道菌群以变形菌门、厚壁菌门及拟杆菌门为主,出生第3~5天时高胆红素血症组及对照组新生儿肠道菌群以变形菌门、厚壁菌门及拟杆菌门为主。与对照组相比,出生24 h内高胆组新生儿肠道埃希氏杆菌属丰度高(P<0.05)。结论出生24 h内高胆红素血症患儿肠道菌群在属水平上以肠道埃希氏杆菌属为主。相比于健康足月母乳喂养新生儿,高胆红素血症新生儿的肠道菌群结构转变较慢,物种多样性及丰度增加缓慢。Objective To observe the structural changes of intestinal flora in breast-fed full-term neonates with hy⁃perbilirubinemia.Methods According to the occurrence of hyperbilirubinemia,40 breast-fed full-term neonates were divided into 12 cases of hyperbilirubinemia(hyperbilirubinemia group)and 28 cases of non-hyperbilirubinemia(control group).The first fetal stool within 24 h of birth and fecal samples from the third to fifth days of birth were respectively col⁃lected from the two groups,16S rDNA gene sequencing technology was used to detect the intestinal flora,βdiversity was used to analyze the species diversity of intestinal flora in the two groups,and key species differences were used to analyze the composition differences of intestinal flora in the two groups.Results The results ofβdiversity of intestinal flora in the hyperbilirubinemia group and control group within 24 hours of birth were 0.51(0.44,0.61)and 0.53(0.42,0.65).The results ofβdiversity of intestinal flora in hyperbilirubinemia group and control group were 0.41(0.28,0.49)and 0.41(0.32,0.52)respectively on the 3rd to 5th days of birth.Compared with those within 24 h of birth,theβdiver⁃sity of intestinal flora in the control group was higher on the 3rd to 5th days of birth(P<0.05).At the genus level,the intestinal floras of neonates in the hyperbilirubinemia group within 24 h of birth were dominated by Escherichia,Aquabacte⁃riu and Pelomonas,while those in the control group were dominated by Aquabacteriu,Pelomonas and Clostridium sensu stricto;on the 3rd to 5th days of birth,the intestinal floras of neonates in the hyperbilirubinemia group were dominated by Escherichia,Clostridium sensu stricto and Streptococcus,while those in the control group were dominated by Clostridium sensu stricto,Escherichia,and Bacteroides.At the phylum level,the intestinal floras of neonates in the hyperbilirubinemia group and the control group within 24 h of birth were mainly Proteobacteria,Firmicutes and Bacteroidetes,and the intesti⁃nal floras of neonates in

关 键 词：肠道菌群 新生儿黄疸 高胆红素血症 埃希氏杆菌属 

分 类 号：R717[医药卫生—妇产科学]