小儿复方鸡内金咀嚼片对KM小鼠全身过敏反应、大鼠腹腔肥大细胞和RBL-2H3细胞类过敏反应诱发结果  

Induction results of CCEC on systemic allergic reactions in KM mice,and anaphylactoid reaction in rat peritoneal mast cells and RBL-2H3 cells

作　　者：郭中华 李更生[2] 其木格 马爽 委亚鸽 李宁 GUO Zhonghua;LI Gengsheng;QI Muge;MA Shuang;WEI Yage;LI Ning(Henan Taloph Pharmaceutical Co.,Ltd.,Zhengzhou 450001,China;不详)

机构地区：[1]河南太龙药业股份有限公司,郑州450001 [2]河南省中医药研究院科教科

出　　处：《山东医药》2024年第18期45-49,共5页Shandong Medical Journal

摘　　要：目的观察小儿复方鸡内金咀嚼片对KM小鼠全身过敏反应、分离自SD大鼠腹腔液肥大细胞(RPMC)和Wistar大鼠嗜碱性白血病细胞株RBL-2H3类过敏反应的诱发结果。方法①取48只KM小鼠,随机均分为甲、乙、丙、丁组:甲组小鼠用小儿复方鸡内金咀嚼片混悬液灌胃,0.25 g生药/kg,隔天1次,连用3次,分别间隔9、16天时再次灌胃;乙组小鼠用小儿复方鸡内金咀嚼片混悬液灌胃,0.025 g生药/kg,隔天1次,连用3次,分别间隔9、16天时再次灌胃;丙组小鼠用1 mg卵清蛋白静脉注射,隔天1次,连用3次,分别间隔9、16天时再次静脉注射;丁组小鼠用0.1 mL/10 g生理盐水静脉注射,隔天1次,连用3次,分别间隔9、16天时再次静脉注射,观察各组小鼠主动全身过敏反应情况。②另取10只SD大鼠,分离大鼠腹腔原代RPMC细胞,分为5组:空白对照、C48/80组、高中低剂量组,高中低剂量组细胞分别加入32、16、8 mg/mL的小儿复方鸡内金咀嚼片混悬液,C48/80组及空白对照组分别加入0.04 mg/mL的组胺释放剂和肥大细胞脱颗粒剂C48/80、缓冲液,测算各组细胞组胺、β-氨基己糖苷酶释放率及促进率。③取对数生长期RBL-2H3细胞,分为空白对照、C48/80组、高中低剂量组,高中低剂量组细胞分别加入32、16、8mg/mL的小儿复方鸡内金咀嚼片混悬液,C48/80组及空白对照组分别加入0.04 mg/mL的C48/80、缓冲液,测算各组细胞组胺、β-氨基己糖苷酶释放率及促进率。结果第14、21天时甲乙丁组小鼠均未出现过敏反应,过敏反应阴性;丙组小鼠出现呼吸困难、死亡,过敏反应强阳性。RPMC细胞C48/80组组胺释放率72.6%(致敏阳性),高中低剂量组的组胺释放促进率均低于64.23%(不具有致敏作用);C48/80组β-氨基己糖苷酶释放促进率为110.53%,(致敏阳性),高中低剂量组的β-氨基己糖苷酶释放促进率均低于80.31%(不具有致敏作用)。RBL-2H3细胞C48/80组的组胺释放率促进率81.6%,高�Objective To observe the induction results of Child Compound Endothelium Comeum(CCEC)on sys⁃temic allergic reactions in KM mice and anaphylactoid reaction in rat peritoneal mast cells(RPMC)and basophilic leuke⁃mia cell line RBL-2H3 in Wistar rats.Methods(1)Forty-eight KM mice were randomly divided into groups A,B,C and D.Mice in the group A were given 0.25g crude drug/kg CCEC suspension,once every other day for three consecutive times,and they were gavaged again at intervals of 9 and 16 days,respectively;mice in the group B were given 0.025 g crude drug/kg CCEC suspension,once every other day for three consecutive times,and they were gavaged again at inter⁃vals of 9 and 16 days,respectively;mice in the group C were given 1 mg ovalbumin(OVA)intravenously,once every oth⁃er day for three consecutive times,and they were gavaged again at intervals of 9 and 16 days,respectively;mice in the group D were given intravenous injection of 0.1 mL/10 g normal saline,once every other day for three consecutive times,and they were gavaged again at intervals of 9 and 16 days,respectively;the active systemic allergic reaction of rats in each group was observed.(2)Primary RPMC were isolated from the abdominal cavity of another 10 SD rats and were divided in⁃to five groups:the blank control group,C48/80 group,high-,medium-and low-dose groups;cells in the high-,medi⁃um-,and low-dose groups were added with 32,16 and 8 mg/mL of CCEC suspension,respectively.The cells of the C48/80 group and blank control group were added with 0.04 mg/mL of histamine release agent and mast cell degranulation agent C48/80 and buffer solution,respectively;the release rate and promotion rate of histamine andβ-amino-hexosidase were measured in each group.(3)RBL-2H3 cells in the logarithmic growth phase were divided into the blank control group,C48/80 group and high-,medium-and low-dose groups.The cells in high-,medium-and low-dose groups were added with 32,16 and 8mg/mL of CCEC suspension,respectively,and the cells in C48/80 group and blank contr

关 键 词：过敏反应 全身过敏反应 类过敏反应 小儿复方鸡内金咀嚼片 肥大细胞 大鼠嗜碱性白血病细胞株 

分 类 号：R285.5[医药卫生—中药学]