通络熄风汤通过调控Mst1/Sirt3信号通路对自发性高血压大鼠心肌损伤程度的影响  

Effect of Tongluo Xifeng Decoction on Myocardial Injury in Spontaneously Hypertensive Rats by Regulating Mst1/Sirt3 Signaling Pathway

作　　者：王继升[1] 李海松[1] 王璐 高昂 冯隽龙 李海燕[1] 吴洋 王显[1] WANG Jisheng;LI Haisong;WANG Lu;GAO Ang;FENG Junlong;LI Haiyan;WU Yang;WANG Xian(Dongzhimen Hospital,Beijing University of Chinese Medicine,Beijing 100700,China)

机构地区：[1]北京中医药大学东直门医院,北京100700

出　　处：《中西医结合心脑血管病杂志》2024年第13期2342-2346,共5页Chinese Journal of Integrative Medicine on Cardio-Cerebrovascular Disease

基　　金：中国博士后创新人才支持计划(No.BX20220047)。

摘　　要：目的:探讨通络熄风汤改善高血压大鼠心肌损伤的作用机制。方法:6只Sprague-Dawley大鼠作为空白组,12只自发性高血压大鼠随机分为模型组和通络熄风汤组。空白组和模型组大鼠给予去离子水灌胃,通络熄风汤组给予中药配方颗粒通络熄风汤悬浮液灌胃治疗,连续干预8周。采用蛋白免疫印迹法(Western Blot)检测心肌细胞中自噬受体蛋白1(SQSTM1/P62)、磷酸化哺乳动物无菌20样激酶1(p-Mst1)、沉默信息调节因子3(Sirt3)蛋白的含量,运用苏木精-伊红(HE)染色观察大鼠心肌组织结构变化,以评价通络熄风汤对高血压心肌损伤改善的效果及机制。结果:与模型组比较,通络熄风汤组能够显著改善高血压大鼠的收缩压及舒张压(P<0.01)。通络熄风汤治疗后大鼠心肌细胞与模型组比较,排列情况与坏死状态均有明显改善。通络熄风汤组大鼠心肌组织中的p-Mst1、P62蛋白含量较模型组降低(P<0.01),Sirt3蛋白含量升高(P<0.01)。结论:通络熄风汤可以介导Mst1/Sirt3信号通路对心肌组织细胞自噬水平进行调整,并且保护心脏功能。Objective:To clarify the therapeutic effect on hypertensive myocardial injury in rats.Methods:Six Sprague-Dawley rats were used as blank group,and 12 spontaneously hypertensive rats were randomly divided into model group and Tongluo Xifeng decoction group.Rats in blank group and model group were given deionized water intragastrically,and Tongluo Xifeng decoction group was given TCM formula granule Tongluo Xifeng decoction suspension intragastrically for 8 weeks.The contents of autophagy receptor protein 1(SQSTM1/P62),phosphorylated mammalian aseptic 20-like kinase 1(p-Mst1)and silence-information regulator 3(Sirt3)protein in cardiomyocytes were detected by Western Blot.The changes of myocardial tissue structure in rats were observed by hematoxylin-eosin(HE)staining to evaluate the effect of Tongluo Xifeng decoction on hypertensive myocardial injury.Results:Compared with model group,Tongluo Xifeng decoction group could significantly improve systolic and diastolic blood pressure(P<0.01).After treatment with Tongluo Xifeng decoction,the arrangement and necrotic state of rat cardiomyocytes significantly improved compared with that of model group.The protein contents of P-MST1 and P62 in myocardial tissue of Tongluo Xifeng decoction group significantly decreased compared with that of model group(P<0.01),while the protein content of Sirt3 significantly increased(P<0.01).Conclusion:Tongluo Xifeng decoction can mediate Mst1/Sirt3 signaling pathway to adjust the autophagy level of myocardial tissue cells and protect cardiac function.

关 键 词：高血压 心肌损伤 通络熄风汤 细胞自噬 哺乳动物无菌20样激酶1/沉默信息调节因子3信号通路 

分 类 号：R285.5[医药卫生—中药学]