艾司氯胺酮对大鼠下肢缺血再灌注后心肌损伤的影响  

Effect of Esketamine on Myocardial Injury after Ischemia Reperfusion of Hindlimb in Rats

作　　者：王迪 王利利[2] WANG Di;WANG Lili(Shanxi Medical University,Taiyuan 030001,Shanxi,China)

机构地区：[1]山西医科大学,太原030001 [2]山西医科大学第二医院,太原030001

出　　处：《中西医结合心脑血管病杂志》2024年第13期2353-2356,共4页Chinese Journal of Integrative Medicine on Cardio-Cerebrovascular Disease

摘　　要：目的:观察艾司氯胺酮对大鼠下肢缺血再灌注(LIR)所诱发的心肌损伤的影响,探索艾司氯胺酮不同给药时间对心肌损伤的影响。方法:将30只SD大鼠随机分为假手术组(Sham组)、缺血再灌注组(IR组)、假手术+艾司氯胺酮组(Sham+Esk组)、缺血前艾司氯胺酮+缺血再灌注组(IR+Esk1组)、再灌注前艾司氯胺酮+缺血再灌注组(IR+Esk2组),每组6只。除假手术组外,其余各组制备下肢缺血再灌注模型(缺血3 h,再灌注2 h)。经药物处理后,采用酶联免疫吸附试验(ELISA)法检测各组大鼠血清肌钙蛋白I(cTnI)、肿瘤坏死因子α(TNF-α)、白细胞介素-6(IL-6)、去甲肾上腺素(NE)含量的变化。结果:相较于Sham组,IR组血清cTnI、TNF-α、IL-6、NE含量升高,Sham+Esk组血清cTnI、TNF-α、IL-6含量降低、NE含量升高(P<0.05)。相较于IR组,IR+Esk1组和IR+Esk2组血清cTnI、TNF-α、IL-6含量降低,IR+Esk1组血清NE含量降低、IR+Esk2组血清NE含量升高,差异有统计学意义(P<0.05)。结论:艾司氯胺酮可能通过抑制炎症反应对大鼠下肢缺血再灌注导致的心肌损伤产生保护作用。Objective:To observe the effects of esketamine on myocardial injury(MI)induced by hindlimb ischemia reperfusion(LIR)in rats,and explore the effects of different administration times of esketamine on myocardial injury.Methods:Thirty SD rats were randomly divided into sham surgery group(Sham group),ischemia reperfusion group(IR group),sham surgery+esketamine group(Sham+Esk group),pre-ischemic esketamine+ischemia-reperfusion group(IR+Esk1 group)and pre-reperfusion esketamine+ischemia-reperfusion group(IR+Esk2 group),with 6 rats in each group.Hindlimb ischemia reperfusion model were prepared in other groups except sham group(ischemia 3 h,reperfusion 2 h).After drug treatment,the contents of troponin I(cTnI),tumor necrosis factorα(TNF-α),interleukin-6(IL-6),and norepinephrine(NE)in serum of each group were measured by enzyme-linked immunosorbent assay(ELISA).Results:Compared with Sham group,the contents of serum cTnI,TNF-α,IL-6,and NE contents increased in IR group,while cTnI,TNF-αand IL-6 contents decreased and NE contents increased in Sham+Esk group(P<0.05).Compared with IR group,the contents of serum cTnI,TNF-αand IL-6 contents in IR+Esk1 group and IR+Esk2 group decreased,serum NE content in IR+Esk1 group decreased,and NE in IR+Esk2 group increased,with statistical significance(P<0.05).Conclusion:Esketamine may show a protective effect on myocardial injury induced by hindlimb ischemia reperfusion in rats by inhibiting inflammatory response.

关 键 词：下肢缺血再灌注 艾司氯胺酮 心肌损伤 炎性因子 实验研究 

分 类 号：R614[医药卫生—麻醉学]