基于γ-氨基丁酸抑制性神经通路探讨“长强”配“太溪”穴电针治疗自闭症神经发育障碍的作用机制  

The Treatment of Autism Neurodevelopmental Disorders with "Changqiang" and "Taixi"Acupoints Electroacupuncture

作　　者：林锦韬[1] 李爱武[1] 刘钊汝[1] 宫凤英[1] 张强[1] 李永春[1] 黄荣铝[1] 吕英[1] 成云水[1] LIN Jintao;LI Aiwu;LIU Zhaoru;GONG Fengying;ZHANG Qiang;LI Yongchun;HUANG Ronglyu;LYU Ying;CHENG Yunshui(Southern Hospital,Southern Medical University,Guangzhou 510515,Guangdong,China)

机构地区：[1]南方医科大学南方医院,广州510515

出　　处：《中西医结合心脑血管病杂志》2024年第13期2357-2362,共6页Chinese Journal of Integrative Medicine on Cardio-Cerebrovascular Disease

基　　金：国家自然青年科学基金项目(No.82105006);2019年广东省中医药局面上项目(No.20191224,20201232);南方医科大学南方医院院长基金项目(No.2019B026,2020B031)。

摘　　要：目的:探讨“长强”配“太溪”穴电针治疗自闭症神经发育障碍仔鼠的靶向调控机制。方法:选取已成功受孕SD大鼠10只,分为造模组和非造模组,每组5只,造模组大鼠给予丙戊酸钠600 mg/kg灌胃造模,非造模组给予等量生理盐水灌胃。将造模组大鼠所产36只仔鼠随机分为电针穴位组、电针非经非穴组和模型组,每组12只,随机选取非造模组仔鼠所产的仔鼠12只作为对照组。电针穴位组仔鼠在出生第24天即开始电针长强和太溪穴位。电针非经非穴组避开经络和穴位针刺。分别于干预前后对所有仔鼠行旷场实验和Morris水迷宫测试。并取仔鼠腹主动脉血检测血清谷氨酸(Glu)、γ-氨基丁酸(GABA)水平。断头处死留取海马组织,通过实时荧光定量聚合酶链式反应(RT-PCR)测定仔鼠海马组织γ-氨基丁酸A型受体(GABAAR)m RNA相对表达水平,采用蛋白免疫印迹法(Western Blot)测定仔鼠海马组织糖原合酶激酶-3β(GSK-3β)、β-catenin和微白蛋白(PV)相对表达水平。结果:旷场实验结果表明,经干预后,电针穴位组水平得分和垂直得分高于模型组和电针非经非穴组,低于对照组,差异有统计学意义(P<0.05)。Morris水迷宫测试结果表明,经干预后,与模型组和电针非经非穴组比较,电针穴位组逃避潜伏期缩短,而穿越次数增加,平台停留时间延长,差异有统计学意义(P<0.05)。与模型组比较,电针穴位组仔鼠Glu、GABA、海马GABAAR m RNA、β-catenin和PV相对表达水平降低,而GSK-3β蛋白相对表达水平上调,差异有统计学意义(P<0.05)。结论:针刺长强和太溪穴可有效改善自闭症仔鼠的空间学习和记忆能力,靶向调控自闭症神经发育障碍,其具体机制可能与通过促进GABA神经通路的兴奋-抑制平衡,同时作用于Wnt/β-catenin信号通路中的重要调节因子有关。Objective:To explore the targeted regulatory mechanism of"Changqiang"combined with"Taixi"acupoints electroacupuncture in the treatment of autistic neurodevelopmental disorders in offspring mice.Methods:Ten SD rats were divided into model group and non-model group,with 5 groups in each group.The rats in the model group were given 600 mg/kg sodium valproate by gavage to establish models,and the rats in the non-model group were given the equal volume of normal saline by gavage.The offspring of the rats in the model group were randomly divided into electroacupuncture acupoint group,electroacupuncture non-meridian non-acupoint group and model group with 12 rats,and 12 offspring rats from the non-model group were randomly selected as the control group.The rats in the electroacupuncture acupoint group started electroacupuncture at Changqiang and Taixi acupoints at the 24th day after birth.The electroacupuncture non-meridian non-acupoint group avoided meridian and acupoint acupuncture.Open field experiment and morris water maze test were performed before and after intervention.The hippocampus was collected by decapitation,and the relative expression level ofγ-aminobutyric acid type A receptor(GABAAR)mRNA in the hippocampus of the offspring were determined by real-time quantitative PCR(RT-PCR).The relative expression levels of glycogen synthase kinase-3β(GSK-3β),β-catenin and microalbumin(PV)were measured by Western Blot.Results:The open field experiment results showed that after intervention,the horizontal and vertical scores of electroacupuncture acupoint group were higher than those of model group and electroacupuncture non-meridian non-acupoint group,but lower than those of the control group(P<0.05).Morris water maze test results showed that after intervention,compared with the model group and the electroacupuncture non-meridian non-acupoint group,the escape latency of the electroacupuncture point group shortened,the crossing times increased,and the platform residence time prolonged(P<0.05).Compared with model gro

关 键 词：自闭症 Γ-氨基丁酸 长强穴 太溪穴 针刺 

分 类 号：R245[医药卫生—针灸推拿学]