NaCl对樱桃砧木组培生根、IAA原位分布及相关酶活性的影响  

Effects of NaCl on rooting,in-situ distribution of IAA and related enzyme activities of cherry rootstock seedlings

作　　者：高兰芸 刘昊 李爱 张婷婷 杨丽芳[2] 高英 GAO Lanyun;LIU Hao;LI Ai;ZHANG Tingting;YANG Lifang;GAO Ying(College of Horticulture and Landscape Architecture,Tianjin Agricultural University,Tianjin 300392,China;Forestry Fruit Research Institute,Tianjin Academy of Agricultural Sciences,Tianjin 300384,China)

机构地区：[1]天津农学院园艺园林学院,天津300392 [2]天津市农业科学院林业果树研究所,天津300384

出　　处：《浙江农业学报》2024年第6期1300-1308,共9页Acta Agriculturae Zhejiangensis

基　　金：国家自然科学基金项目(31800572);天津市现代农业产业技术体系建设(林果)项目(ITTHRS2021000)。

摘　　要：为阐明NaCl对樱桃砧木组培生根的影响,试验以吉塞拉6号组培苗为材料,首先研究了不同浓度NaCl处理下组培苗不定根发生指标,然后根据生根指标进一步研究了10 mmol·L^(-1)和80 mmol·L^(-1)NaCl处理下的解剖结构、发根过程酶活性及吲哚-3-2酸(IAA)的原位分布。结果表明,10 mmol·L^(-1)NaCl处理下樱桃组培苗生根率和生根指数均最高,且显著高于其他处理,分别为58%和13.43;该处理下生根最快,2 d时形成层开始增厚,6 d时根原基形成,10 d时不定根突破表皮。各处理超氧化物歧化酶(SOD)活性在6 d时均达到峰值;过氧化物酶(POD)、多酚氧化酶(PPO)、吲哚乙酸氧化酶(IAAO)活性变化各处理存在差异,CK均呈上升趋势,与CK相比,10 mmol·L^(-1)处理IAAO活性于2 d出现峰值。利用免疫胶体金技术对IAA进行原位分析发现,10 mmol·L^(-1)处理2 d时IAA主要分布于形成层,6 d时IAA大量分布于根原基,10 d时IAA集中分布于不定根维管组织。综上,10 mmol·L^(-1)NaCl处理对樱桃组培苗不定根发生具有一定的促进作用,为进一步研究樱桃组培苗不定根发生及调控机制提供参考。In order to clarify the effect of NaCl on the rooting of cherry rootstock,this experiment took the Gisela 6 tissue culture seedling as the material,and firstly studied the index of adventitious root occurrence in the tissue culture seedling treated with different concentrations of NaCl.Then,the anatomical structure,enzyme activity and in-situ distribution of IAA under 10 mmol·L^(-1) and 80 mmol·L^(-1) NaCl were further studied according to rooting indexes.The results showed that the rooting rate and rooting index of cherry tissue seedlings were the highest under the treatment of 10 mmol·L^(-1) NaCl,and were significantly higher than other treatments,which were 58%and 13.43,respectively.Under this treatment the rooting speed was the fastest,the cambium thickened at 2 d,the root primodia formed at 6 d,and the adventitious roots broke through the epidermis at 10 d.SOD activity of each treatment reached the peak at 6 d.The activity changes of POD,PPO and IAAO were different among all treatments,while the activity of CK showed an increasing trend.Compared with CK,the activity of IAAO in 10 mmol·L^(-1) treatment peaked at 2 d.Using immune colloidal gold technique to analysis the in-situ distribution of IAA,we found under 10 mmol·L^(-1) NaCl treatment the IAA mainly distributed in cambium at 2 d,in root primordium at 6 d,and in the adventitious root vascular tissue at 10 d.In conclusion,10 mmol·L^(-1) NaCl treatment can promote adventitious roots of cherry tissue culture seedlings,and provide a reference for further research on the occurrence and regulation mechanism of adventitious roots.

关 键 词：樱桃 砧木 生根 酶活性 免疫组化 

分 类 号：S662.5[农业科学—果树学]