长江中下游小麦新品系条锈病抗性评估与抗病基因分析  

Evaluation of Stripe Rust Resistance and Analysis of Disease Resistance Genes of theNew Wheat Lines in the Middle and Lower Reaches of the Yangtze River

作　　者：李壮 黄彦川 张传量 蔚睿 刘胜杰 吴建辉 李春莲[1] 郑炜君[1] 曾庆东[2] 康振生[2] 韩德俊[1] LI Zhuang;HUANG Yanchuan;ZHANG Chuanliang;YU Rui;LIU Shengjie;WU Jianhui;LI Chunlian;ZHENG Weijun;ZENG Qingdong;KANG Zhensheng;HAN Dejun(College of Agronomy,Northwest A&F University/State Key Laboratory For Crop Stress Resistance and High-Efficiency Production,Yangling,Shaanxi 712100,China;College of Plant Protection,Northwest A&F University/State Key Laboratory For Crop Stress Resistance and High-Efficiency Production,Yangling,Shaanxi 712100,China)

机构地区：[1]西北农林科技大学农学院/作物抗逆与高效生产全国重点实验室,陕西杨凌712100 [2]西北农林科技大学植物保护学院/作物抗逆与高效生产全国重点实验室,陕西杨凌712100

出　　处：《麦类作物学报》2024年第7期835-845,共11页Journal of Triticeae Crops

基　　金：国家十四五重点研发计划项目(2021YFD1200602)。

摘　　要：由小麦条锈菌(Puccinia striiformis West. f. sp.tritici Eriks.&Henn.)引起的条锈病是世界范围内小麦的重要病害。长江中下游麦区是中国小麦主产区之一和重要的小麦商品粮基地,也是中国小麦条锈病流行的重点防控地区。准确评价该地区小麦品种(系)对当前小麦条锈病流行小种的抗性,了解抗条锈基因在各个品种及所处麦区内的分布状况,对及时调整抗病育种目标、指导抗病品种及抗病基因的合理布局与应用具有重要意义。本研究收集了2019-2021年间苏、皖、鄂等长江中下游麦区103份小麦材料,包括34份当地主栽品种和69份参加新品种区域审定试验的高代品系,于2021年分别在陕西杨凌混合小种(CYR32、V26-lab)人工病圃及四川江油自然诱发病圃进行成株期抗条锈性鉴定;以当前流行条锈菌小种条中32(CYR32)和G22致病类群(V26-lab)对参鉴品系进行苗期分小种鉴定,利用16个已知抗条锈基因的19个SNP标记进行分子检测,其中包括11个全生育期抗病基因[Yr5a、Yr5b (YrSP)、Yr6、Yr7、Yr9、Yr15、Yr26、Yr28、Yr81、YrZH84和YrAS1676]和5个成株期抗病基因(Yr18、Yr30、Yr78、Yr86和YrLT26),推测小麦品系可能携带的抗病基因。结果表明,34份主栽品种中,23份表现成株期抗病。在69份高代品系中,苗期对CYR32和V26-lab小种表现抗病的品系分别有11份和13份,对CYR32和V26-lab均表现免疫或者近免疫的品系仅有2份。根据品系成株期抗病DS值的聚类热图结果表明,在杨凌表现抗病的品系有44份,在江油表现抗病的品系有25份,在两地均表现环境稳定抗病的品系有17份。分子检测表明,参鉴品系中可能携带Yr5b、Yr6、Yr7、Yr9、Yr30、Yr78、Yr81、Yr86、YrZH84、YrLT26和YrAS1676,其中只有Yr6、Yr81和YrLT26显著影响抗病性,没有发现可能携带Yr5a、Yr15、Yr18、Yr26和Yr28的品系。此外,Yr78、Yr81、Yr86和YrAS1676组合表现可有效抗条锈病。长江中下游麦区�Stripe rust or yellow rust(YR)caused by Puccinia striiformis West.f.sp.tritici Eriks.&Henn.(Pst)is an important disease of wheat worldwide.The wheat growing region in the middle and lower reaches of the Yangtze River is one of the main wheat production areas and the important commodity grain base in China.It is also a severe YR endemic region in China.Evaluation of the resistance to YR for wheat varieties(lines)and characterization the corresponding resistance genes in the region is of great significance for disease control.However,no information is available regarding YR resistance and the genes in wheat breeding lines in this region.In this study,103 wheat accessions comprised of 34 elite commercial varieties and 69 advanced breeding lines were collected from this region including Jiangsu,Anhui,and Hubei from 2019 to 2021.In 2021,adult plant resistance(APR)was evaluated in the artificial infection nursery with mixed races(CYR32,V26-lab)at Yangling in Shaanxi and the naturally induced disease nursery at Jiangyou in Sichuan.The current prominent Pst races CYR32 and V26-lab were used for seedling test.A total of 19 SNP markers related to 16 resistance genes were used for molecular detection,including 11 all-stage resistance(ASR)genes(Yr5a,Yr5b,Yr6,Yr7,Yr9,Yr15,Yr26,Yr28,Yr81,YrZH84,and YrAS1676)and 5 APR genes(Yr18,Yr30,Yr78,Yr86,and YrLT26).Result showed that 23 out of the 34 elite varieties exhibited APR at both Yangling and Jiangyou.Among the 69 advanced lines,11 and 13 lines conferred resistance to CYR32 and V26-lab at seedling stage,respectively,among which only 2 lines showed near immunity to both CYR32 and V26-lab.Cluster analysis of disease severity for wheat lines indicated that there were 44 lines and 25 lines conferring APR at Yangling and Jiangyou,respectively,and only 17 lines displayed stable resistance in both environments.Molecular detection showed that the tested lines may carry Yr5b,Yr6,Yr7,Yr9,Yr30,Yr78,Yr81,Yr86,YrZH84,YrLT26,and YrAS1676,yet Yr5a,Yr15,Yr18,Yr26,or Yr28 were not found in these

关 键 词：小麦条锈病 Yr基因 SNP标记 小麦抗条锈育种 长江中下游麦区 

分 类 号：S512.1[农业科学—作物学] S330