贝莱斯芽胞杆菌LQ-3防治小麦纹枯病的效果及根际定殖能力  

Biocontrol Effect of Bacillus velezensis LQ-3 against Wheat SharpEyespot and Its Colonization Dynamics in the Rhizosphere of Wheat

作　　者：李蓉 洪赫 姜文筱 王艳斌 张红娟[1] 赵芳 刘杰辉 张泽 梁艳[1] 王春静 谢咸升 LI Rong;HONG He;JIANG Wenxiao;WANG Yanbin;ZHANG Hongjuan;ZHAO Fang;LIU Jiehui;ZHANG Ze;LIANG Yan;WANG Chunjing;XIE Xiansheng(Institute of Wheat Research,Shanxi Agricultural University,Linfen,Shanxi 041000,China;College of Plant Protection,China Agricultural University,Beijing 100193,China)

机构地区：[1]山西农业大学小麦研究所,山西临汾041000 [2]中国农业大学植物保护学院,北京100193

出　　处：《麦类作物学报》2024年第7期947-954,共8页Journal of Triticeae Crops

基　　金：山西省基础研究计划(自由探索类)青年项目(20210302124679);山西省现代农业产业技术体系建设专项资金(2023CYJSTX02-18);农业基础性长期性科技工作农用微生物观测监测任务(NAES-AM-009);农业基础性长期性科技工作植物保护监测(NAES-PP-017)。

摘　　要：小麦纹枯病(wheat sharp eyespot)主要是由禾谷丝核菌(Rhizoctonia cerealis)侵染引起的一种土传真菌病害,严重威胁小麦生产安全。目前,利用生防细菌防治小麦纹枯病已成为研究热点,其中,研究最多的一类生防细菌为芽胞杆菌。为明确贝莱斯芽胞杆菌(Bacillus velezensis)LQ-3防治小麦纹枯病的效果及其在小麦根部定殖能力,本研究利用LQ-3发酵液灌根处理小麦苗,调查统计LQ-3对小麦纹枯病的防效。同时利用绿色荧光蛋白标记菌株LQ-3(pGFP78)灌根处理小麦根部,并结合抗生素平板回收检测、激光共聚焦显微镜观察方法,探明LQ-3在小麦根部的定殖动态。结果表明,LQ-3防治小麦纹枯病的效果为57.49%,与化学药剂27%苯醚·咯·噻虫悬浮种衣剂(酷拉斯)的防效(59.95%)相当。LQ-3(pGFP78)的生长趋势与野生型LQ-3相比并没有显著性差异,且仍具有游动性和生物膜形成能力。LQ-3(pGFP78)发酵液初始浓度为2.3×10^(8) cfu·mL^(-1),灌根处理5 d后,其在小麦根部的定殖量已达到9.63×10^(6)cfu·g^(-1),25 d后,定殖量仍有1.33×10^(6)cfu·g^(-1),表明LQ-3在小麦根部具有较好的定殖能力。Wheat sharp eyespot,primarily caused by the soil-borne fungal pathogen Rhizoctonia cerealis,poses a severe threat to wheat production security.Currently,the utilization of biocontrol bacteria for the prevention and control of wheat sharp eyespot has emerged as a research hotspot,with Bacillus being the most extensively studied biocontrol bacteria.To determine the biocontrol effect of Bacillus velezensis LQ-3 on wheat sharp eyespot and its colonization ability in wheat root,this study utilized LQ-3 fermentation to irrigate wheat root,aiming to investigate the biocontrol effect of LQ-3 against wheat eye spot.The colonization dynamics of LQ-3 in wheat root were elucidated by using green fluorescent protein labeled strain LQ-3(pGFP78)to irrigate wheat root,combined with antibiotic plate recovery detection and laser confocal microscopy observation.The results showed that the biocontrol effect of LQ-3 on wheat sharp eyespot is 57.49%,which is equivalent to the biocontrol effect of the Kuras at 59.95%.Moreover,the growth trend of LQ-3(pGFP78)exhibited no significant disparity when compared to the wild-type LQ-3,while still retaining its motility and biofilm-forming abilities.The initial concentration of LQ-3(pGFP78)fermentation broth was 2.3×10^(8) cfu·mL^(-1),and after 5 d of root irrigation treatment,the colonization of LQ-3(pGFP78)in wheat root reached 9.63×10^(6) cfu·g^(-1);even after 25 d,the colonization quantity still remained at 1.33×10^(6) cfu·g^(-1),indicating that LQ-3 has proficient colonization capability in wheat root.

关 键 词：贝莱斯芽胞杆菌LQ-3 小麦纹枯病 定殖 防效 

分 类 号：S512.1[农业科学—作物学] S435.12