一种槟榔芋疫病病原菌的分离鉴定与生物学特性  

Isolation,identification and biological characteristics of a pathogenic fungus causing areca taro disease

作　　者：董晓菲[1,2] 王同帅 匡云波[1,2] 叶祖云[1,2] DONG Xiao-fei;WANG Tong-shuai;KUANG Yun-bo;YE Zu-yun(College of Biological Science and Engineering,Ningde Normal University,Ningde,Fujian 352100,China;Engineering Technology Research Center of Characteristic Medicinal Plants of Fujian,Ningde Normal University,Ningde,Fujian 352100,China)

机构地区：[1]宁德师范学院生物科学与工程学院,福建宁德352100 [2]宁德师范学院福建省特色药用植物工程技术研究中心,福建宁德352100

出　　处：《宁德师范学院学报（自然科学版）》2024年第2期168-173,216,共7页Journal of Ningde Normal University(Natural Science)

基　　金：福建省自然科学基金项目(2022J011210);宁德师范学院中青年科研项目(2022ZQ108)。

摘　　要：为提高槟榔芋产量和品质,探究槟榔芋疫病病原菌的生物学特性,从福鼎槟榔芋疫病发病叶片的病斑中分离病原真菌,得到可疑菌株T5-1.经Koch’s法则证病、形态学和测序鉴定,初步证明其属于小孢根霉菌(Rhizopus microsporus).生物学特性研究发现:其菌落正面为灰白色,反面为黄褐色,菌丝毛绒状延展,边缘整齐,黑色孢子囊.实验结果表明,该病原菌在pH=5~9的培养基上均能生长,最适为pH=6,培养温度在4~45℃时均能生长,最适温度为40℃.In order to improve the yield and quality of areca taro,and to explore the biological characteristics of the pathogen of areca taro disease,pathogenic fungi were isolated from spots of the diseased leaves of Fuding areca taro,and the suspected strain T5-1 was obtained.Through Koch’s rule for disease identification,mor⁃phology,and sequencing,it was preliminarily proved that it belongs to Rhizopus microsporus.Research on bio⁃logical characteristics has found that the front of its colony is grayish-white,while the back is yellowish-brown.The hyphae are fluffy and extends,with neat edges and black spore sacs.The experimental results show that the pathogen can grow in the medium of pH=5-9,with an optimal pH=6.It can also grow at 4-45℃,with an optimal temperature of 40℃.

关 键 词：槟榔芋 真菌 小孢根霉菌 生物学特性 

分 类 号：S436.32[农业科学—农业昆虫与害虫防治]