旱地小麦区试品系中抗旱高产相关基因的KASP标记检测  

KASP Marker Detection of Genes Related to Drought Resistance and High Yield in Dryland Regional Trial Wheat Lines

作　　者：高洁[1] 王富延 宋国琦[1] 孔淑鑫 李玉莲[1] 张淑娟[1] 张荣志[1] 李吉虎 李根英[1] 刘鹏 李玮[1] GAO Jie;WANG Fuyan;SONG Guoqi;KONG Shuxin;LI Yulian;ZHANG Shujuan;ZHANG Rongzhi;LI Jihu;LI Genying;LIU Peng;LI Wei(Crop Research Institute,Shandong Academy of Agricultural Sciences/Key Laboratory of Wheat Biology and Genetic Improvement in the North Yellow&Huai River Valley,Ministry of Agriculture and Rural Affairs/National Engineering Laboratory for Wheat&Maize,Ji'nan 250100,China;Dezhou Academy of Agricultural Sciences,Dezhou 253015,China;School of Agricultural Science and Technology,Shandong Agriculture and Engineering University,Dezhou 251100,China)

机构地区：[1]山东省农业科学院作物研究所/农业农村部黄淮北部小麦生物学与遗传育种重点实验室/小麦玉米国家工程实验室,山东济南250100 [2]德州市农业科学研究院,山东德州253015 [3]山东农业工程学院农业科技学院,山东德州251100

出　　处：《山西农业科学》2024年第4期9-15,共7页Journal of Shanxi Agricultural Sciences

基　　金：山东省农业良种工程项目(2021LZGC009,2022LZGCQY002,2021LZGC013);山东省农业科学院创新工程项目(CXGC2023A01)。

摘　　要：利用分子标记检测小麦区试品系,明确其优异等位基因组成,有利于进一步精准改良利用。分析KASP标记检测中存在的问题,有助于促进分子育种应用。以山东省2021—2022年小麦旱地区试24份参试品系为材料,利用32个抗旱高产相关基因的KASP标记,分析KASP标记检测中存在的异常结果和材料的优异等位基因利用情况。结果表明,未知基因型产生的主要原因是KASP反应失败或非特异扩增。混合DNA样品检测结果为杂合的主要原因是单株间基因型不一致,次要原因是错误分型。32个KASP标记中,14个标记的优异等位基因检出率超过83%,表明这些基因在育种中已经得到有效利用;17个标记的优异等位基因检出率低于50%,其中,WRKY51-2B、AX-95025477-7B、SnRK2.4A3、TaMoc-2433和NCED15个标记的优异等位基因未检出,表明这些基因在育种中利用较少,可通过分子标记辅助选择开展遗传改良。24份材料中,HQ04携带20个优异等位基因,是数量最多的,可作为基因供体用于育种。利用KASP标记开展辅助选择定向精准提高优异等位基因在小麦育种中的利用率仍有可为。To detect wheat regional trail lines with molecular markers and clarify favorable allele composition is conducive to the further precise improvement and utilization.Analyzing the problems in KASP marker detection will promote application in molecular breeding.In this study,using twenty-four advanced lines participated the 2021-2022 wheat dryland regional trial of Shandong province as materials,a total of 32 KASP markers associated with drought resistance and high yield were used to analyze the abnormal results in KASP marker detection and utilization of the favorable allele.The results indicated that the main reason for unknown genotypes was KASP reaction failure or non-specific amplification.The main reason for heterozygous results of mixed DNA samples was genotypic inconsistency among individuals,and the secondary reason was genotyping error.Among the 32 markers detected,the favorable allele detection rate of 14 KASP markers was above 83%,indicating that they were already efficiently applied in breeding programs,and the detection rate of the favorable alleles of 17 markers was below 50%.Among the 17 markers,the favorable alleles of the five markers including WRKY51-2B,AX-95025477-7B,SnRK2.4A3,TaMoc-2433,and NCED1 were not detected,indicating that the genes were applied less in breeding.Those less applied genes could be utilized through marker assisted selection for future genetic improvement.Among the 24 tested lines,HQ04 carried 20favorable alleles which was the most and it could be used as a gene donor for breeding.There is still room for accurate and directive improvement of utilization rate of favorable alleles in wheat breeding through KASP marker-assisted selection.

关 键 词：小麦 抗旱 功能基因 KASP标记 区试品系 

分 类 号：S512.1[农业科学—作物学]