Py/GC-MS法检测安全套润滑剂遗留微量物证  

Detection of Trace Evidence of Lubricants in Condoms by Py/GCMS Method

作　　者：陈麒全 赵鹏程[1] 宋辉[1] 赵亮 CHEN Qi-quan;ZHAO Peng-cheng;SONG Hui;ZHAO Liang(Department of Forensic Science and Technology,Criminal Investigation Police University of China,Shenyang 110854,China)

机构地区：[1]中国刑事警察学院公安技术法化学系,辽宁沈阳110854

出　　处：《分析测试学报》2024年第7期995-1002,共8页Journal of Instrumental Analysis

摘　　要：采用裂解气相色谱(Py/GC-MS)建立了安全套润滑剂遗留微量物证的高灵敏检测方法。制作模拟性侵案件样本,用正己烷进行超声提取,过膜处理后在N2流下蒸发提取物至50μL,将收集的提取物用移液枪转移到热解坩埚,待溶剂完全蒸发后,通过Py/GC-MS进行测定。方法学考察结果表明,该方法的定量下限为5μg/kg,回收率为91.0%~103%,相对标准偏差(RSD)为7.1%~8.3%。采用所建方法对聚二甲基硅氧烷(PDMS)标准品和网购销量前20名的安全套制作的模拟样本进行分析,考察了生物流体(血液、尿液、唾液等体液)的干扰和在不同介质(内衣、皮肤)的持久性。该方法从所有硅基润滑安全套样本中均检出PDMS,可排除空白样本的干扰。生物流体对PDMS检测不会产生干扰,在内衣、皮肤环境下的最长附着时间分别为72、48 h,确立了性侵案件发生后的检测时间窗口,为安全套润滑剂遗留微量物证的检测提供了一种准确可靠的方法。In this study,a highly sensitive detection method for trace evidence of condom lubricants was established using Py/GC-MS.Simulated sexual assault case samples were prepared,extracted using hexane ultrasonication,processed through membrane filtration,and evaporated under N2 flow to 50μL.The collected extract was transferred to a pyrolysis crucible using a pipette,evaporated sol⁃vent completely,and analyzed by Py/GC-MS.Methodological investigations showed that the quanti⁃tative limit of this method was 5μg/kg,with recoveries of 91.0%to 103%and relative standard devia⁃tions(RSDs)of 7.1%to 8.3%.The method was used to analyze PDMS standards and simulated sam⁃ples made from the top 20 best-selling condoms from online sellers,assessing interference from bio⁃logical fluids(blood,urine,saliva)and the persistence on different media(underwear,skin).PD⁃MS was detected in all silicone-based lubricated condom samples,excluding interference from blank samples.Biological fluids did not interfere with PDMS detection,and the longest attachment times in underwear and skin environments were 72 hours and 48 hours,respectively,establishing a detection time window after sexual assault incidents.This method provides an accurate and reliable way to de⁃tect trace evidence of condom lubricants.

关 键 词：安全套 润滑油 聚二甲基硅氧烷 Py/GC-MS 持久性 

分 类 号：O657.7[理学—分析化学] D919.2[理学—化学]