盐碱土壤中唐古特白刺内生真菌分离与鉴定  

Isolation and Identification of Endophytic Fungi in Nitraria tangutorum Bobr.in Saline Soil

作　　者：刘艳萍 张得芳[1,2] 文怀秀 LIU Yanping;ZHANG Defang;WEN Huaixiu(Qinghai Academy of Agriculture and Forestry,Qinghai University,Xining 810016,China;Laboratory for Research and Utilization of Qinghai Plateau Germplasm Resources,Xining 810016,China;Qinghai Provincial Key Laboratory of Tibetan Medicine Research and Key Laboratory of Tibetan Medicine Research,Northwest Institute of Plateau Biology,Chinese Academy of Sciences,Xining 810008,China)

机构地区：[1]青海大学农林科学院,西宁810016 [2]青海高原种质资源研究与利用实验室,西宁810016 [3]中国科学院西北高原生物研究所青海省藏药研究重点实验室,西宁810008

出　　处：《西北农业学报》2024年第7期1375-1385,共11页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：国家自然科学基金(32170239);青藏高原种质资源研究与利用实验室(2022-ZJ-Y01)。

摘　　要：为探明唐古特白刺(Nitraria tangutorum Bobr.)的内生真菌种属及其组织分布特性,采用传统组织块法从生长于盐碱土壤的白刺中分离内生真菌,运用形态学和真菌ITS序列分析进行种属鉴定,并通过MEGA7.0构建系统发育树。结果显示,从白刺(根、茎、叶)分离得到23株内生真菌,共鉴定出21株,其中链格孢属(Alternaria)13株,毛壳菌属(Chaetomium)1株,篮状菌属(Talaromyces)3株,细基格孢属(Ulocladium)4株,主要分布于唐古特白刺的根部,叶次之,茎则无。表明白刺内生真菌分布具有组织差异,根是其侵染和定殖的主要部位。To explore the species and tissue distribution charateristics of endophytic fungi in Nitraria tangutorum Bobr.,we isolated fungi from N.tangutorum Bobr.growing in saline soil by traditional tissue block method.The species were identified by morphology and fungal ITS sequence analysis,and a phylogenetic tree was constructed by MEGA 7.0.The results showed that 23 strains of endophytic fungi were isolated from different parts of N.tangutorum Bobr.(roots,stems,leaves),with 21 strains identified.These included thirteen strains of Alternaria,one strain of Chaetomium three strains of,Talaromyce,and four strains of Ulocladium.The predominant distribution was observed in,the roots of N.tangutorum Bobr.followed by leaves,and stems.The results suggest distinct tissue-based difference in the distribution of endophytic fungi,with the root being the most vulnerable infection and colonization.

关 键 词：唐古特白刺 内生真菌 分离 鉴定 盐碱土壤 

分 类 号：S793.9[农业科学—林木遗传育种]