痰热清注射液治疗慢性阻塞性肺病急性加重期作用机制探讨  

Study on the Mechanism of Tanreqing Injection in Treating Acute Exacerbation of Chronic Obstructive Pulmonary Disease

作　　者：谭标标 任薇[2] TAN Biaobiao;REN Wei(Anhui University of Chinese Medicine,Anhui Hefei 230031,China;The First Affiliated Hospital of Anhui University of Chinese Medicine,Anhui Hefei 230031,China)

机构地区：[1]安徽中医药大学,安徽合肥230031 [2]安徽中医药大学第一附属医院,安徽合肥230031

出　　处：《中医药临床杂志》2024年第6期1081-1087,共7页Clinical Journal of Traditional Chinese Medicine

基　　金：安徽省中医药事业传承与发展专项资金项目(皖卫中医药发[2020]5号)。

摘　　要：目的:研究痰热清注射液治疗慢性阻塞性肺病急性加重期作用机制。方法:在线查询TCMSP数据库、既往文献获取黄芩、熊胆粉、山羊角、金银花、连翘等中药的主要活性成分,利用swisstargetprediction数据库获取其活性成分对应靶点;利用GeneCards、OMMI、Drug Bank等数据库获取慢性阻塞性肺病急性加重期靶点。利用Venny平台在线获取药物与疾病靶点交集,通过DAVID数据库对相交靶点进行基因本体分析和通路富集分析,使用STRING数据库构建蛋白相互作用网络,借助Cytoscapr软件行可视化处理并筛选主要活性成分、关键靶点。最后通过AutoDock vina软件将核心靶点与核心成分进行分子对接验证,并利用pymol可视化处理。结果:痰热清注射液中含主要活性成分90个,对应靶点557个;连翘、黄芩、金银花为痰热清注射液的关键作用药物;baicalein、Norwogonin、5,7,4’-Trihydroxy-8-methoxyflavone、5,2’,6’-Trihydroxy-7,8-dimethoxyflavone、(2R)-7-hydroxy-5-methoxy-2-phenylchroman-4-one、Panicolin、Skullcapflavone II、Moslosooflavone、5,7,2,5-tetrahydroxy-8,6-dimethoxyflavone、quercetin等为核心活性成分;STAT3、HSP90AA1、ESR1、SRC、EP300、AKT1、JUN、PIK3R1、RELA等靶点为蛋白互作网络中的核心靶点。结论:痰热清注射液治疗主要通过黄酮类物质调节细胞炎症反应、细胞增殖、凋亡等对慢性阻塞性肺病急性加重期发挥潜在作用。Objective:To study the mechanism of Tanreqing injection in the treatment of acute exacerbation of chronic obstructive pulmonary disease.Methods:Online query of the TCMSP database and previous literature to ob-tain the main active ingredients of traditional Chinese medicines such as skullcap,bear bile powder,goat horn,hon-eysuckle,and forsythia suspensa,and use the swisstargetprediction database to obtain the targets corresponding to the active ingredients;use GeneCards,OMMI,Drug Bank and other databases Obtain targets for acute exacerbation of chronic obstructive pulmonary disease.Use the Venny platform to obtain the intersection of drug and disease targets online,use the DAVID database to conduct gene ontology analysis and pathway enrichment analysis of the intersecting targets,use the STRING database to construct a protein interaction network,and use the Cytoscapr software to visualize and screen the main active ingredients,Key targets.Finally,the core targets and core components were verified by mo-lecular docking through AutoDock vina software,and pymol was used for visual processing.Results:Tanreqing injec-tion contains 90 main active ingredients,corresponding to 557 targets;Forsythia suspensa,skullcap,and honeysuckle are the key drugs of Tanreqing injection;baicalein,Norwogonin,5,7,4’-Trihydroxy-8-methoxyflavone,5,2’,6’-Tri-hydroxy-7,8-dimethoxyflavone,(2R)-7-hydroxy-5-methoxy-2-phenylchroman-4-one,Panicolin,Skullcapflavone II,Moslosooflavone,5,7,2,5-tetrahydroxy-8,6-dimethoxyflavone,quercetin,etc.are core active ingredients;STAT3,HSP90AA1,ESR1,SRC,EP300,AKT1,JUN,PIK3R1,RELA and other targets are core targets in the protein interac-tion network point.Conclusion:Tanreqing injection therapy mainly plays a potential role in the acute exacerbation of chronic obstructive pulmonary disease by regulating cellular inflammatory response,cell proliferation,and apoptosis through flavonoids.

关 键 词：痰热清注射液 慢性阻塞性肺病急性加重期 网络药理学 分子对接技术 作用机制 

分 类 号：R256.1[医药卫生—中医内科学]